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Tools and Resources Environmental DNA

Environmental DNA sampling methods vary depending on the unique ecological conditions and goals of individual sampling programs. Peer-reviewed publications usually include summaries of the specific methods used for sample collection, concentration, and preservation, but a handful of published protocols provide more detailed, step-by-step instructions that can be used in many sampling situations. A critical component of these and other sampling protocols is strict adherence to practices for preventing cross-contamination among samples. If you’re modifying existing protocols for a new aquatic system or sampling objectives, continue to follow stringent measures to avoid contamination.

Washington State University

WSU eDNA sampling protocol Jan2017

Goldberg, Caren, and Katherine Strickler. 2016. Washington State University, Pullman, WA. 15p.

Rocky Mountain Research Station

A protocol for collecting environmental DNA samples from streams. Rocky Mountain Research Station 

Carim, Kellie J.; McKelvey, Kevin S.; Young, Michael K.; Wilcox, Taylor M.; Schwartz, Michael K. 2016. A protocol for collecting environmental DNA samples from streams. Gen. Tech. Rep. RMRS-GTR-355. Fort Collins, CO: U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station. 18 p.

U.S. Geological Survey

Environmental DNA sampling protocol – filtering water to capture DNA from aquatic organisms

Laramie, M.B., Pilliod, D.S., Goldberg, C.S., and Strickler, K.M., 2015, Environmental DNA sampling protocol— Filtering water to capture DNA from aquatic organisms: U.S. Geological Survey Techniques and Methods, book 2, chap. A13, 15 p., http://dx.doi.org/10.3133/tm2A13.