{"id":2,"date":"2016-04-27T00:02:45","date_gmt":"2016-04-27T00:02:45","guid":{"rendered":"http:\/\/labs.wsu.edu\/flowcore\/?page_id=2"},"modified":"2019-05-23T10:50:10","modified_gmt":"2019-05-23T17:50:10","slug":"home","status":"publish","type":"page","link":"https:\/\/labs.wsu.edu\/flowcore\/","title":{"rendered":"Home"},"content":{"rendered":"
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Flow Cytometry<\/h1>\n<\/p><\/div>\n<\/section>\n
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Flow Cytometry is a highly quantitative high throughput technique to accurately detect and quantitate various cell components at the single cell level.<\/p>\n<\/p><\/div>\n

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Contact Us<\/h1>\n

Ze Liu<\/strong>
\nResearch Supervisor
\n509-358-7633
\nze.liu@wsu.edu<\/a><\/p>\n<\/p><\/div>\n<\/section>\n

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Bio-Rad S3 Cell Sorter<\/h2>\n<\/header>\n

This automated benchtop cell sorter is equipped with 2 lasers, 4 fluorescense detectors, and forward- and side-scatter detectors. Features of the unit include simplified instrument setup, reduced carryover, 2-way cell sorting, high speed and high purity, and intuitive interface.<\/p>\n\n\n\n\n\t\n\n\t
Excitation Laser<\/th>Acquisition Channel\/Filter<\/th>Example Fluorescent Proteins<\/th>\n<\/tr>\n<\/thead>\n
488 nm
\n561 nm
\nCo-linear<\/td>		FI1 \u2013 525\/30
\nFI2 \u2013 586\/25
\nFI3 \u2013 615\/25
\nFI4 \u2013 655LP<\/td>		FitC, AF488, GFP, PE, PE-TR,
\nPI, mCherry from 561nm, PerCEP, PE-cy7<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n<\/p><\/div>\n<\/section>\n
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Beckman Coulter Gallios<\/h2>\n<\/header>\n

This unit is a research system that delivers analytical excellence by coupling extraordinary sensitivity resolution and dynamic range with high speed data collection. Along with excellent detection capabilities, the Gallios includes easy to use software and automation to facilitate superior performance of multi-color flow cytometry assays.<\/p>\n\n\n\n\n\t\n\n\t
Excitation Laser<\/th>Acquisition Channel\/Filter<\/th>Example Fluorescent Proteins<\/th>\n<\/tr>\n<\/thead>\n
488 nm
\n638 nm<\/td>		FI1 \u2013 525\/40
\nFI2 \u2013 575\/30
\nFI3 \u2013 620\/30
\nFI4 \u2013 695\/30
\nFI5 \u2013 755LP
\nFI6 \u2013 660\/20<\/td>		FitC, AF488, GFP, PE, PE-TR, PI, PerCP, PE-Cy5.5, PE-Cy7, PE-AF700, APC, AF647<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n<\/p><\/div>\n<\/section>\n
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Becton Dickinson Accuri C6<\/h2>\n<\/header>\n

This unit is equipped with 2 lasers, 2 light scatter detectors, and 4 fluroescense detectors with optical filters optimized for the detrection pf FITC, PE, PerCP, APC. Data is digitally collected over a wide dynamic range of 7.2 decades, making all data available to users as needed.<\/p>\n\n\n\n\n\t\n\n\t
Excitation Laser<\/th>Acquisition Channel\/Filter<\/th>Example Fluorescent Proteins<\/th>\n<\/tr>\n<\/thead>\n
488 nm
\n640 nm<\/td>		FI1 \u2013 510\/15
\n530\/30
\nFI2 \u2013 585\/40
\n540\/20
\nFI3 \u2013 670LP
\n610\/20
\nFI4 \u2013 675\/25<\/td>		GFP, FitC, AF488,
\nPE, PI, YFP (bleeds into 530),
\nPerCP, PE-cy5.5, PI, PE-TR, APC, AF647, AF700<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n<\/p><\/div>\n<\/section>\n
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Software<\/h2>\n<\/header>\n

Each cytometer has its own acquisition software, specifically designed to optimally run the machine and acquire data. All files can be saved as general format FCS files and further analyzed with Beckman Coulter Kaluza analysis software available for use in the CORE.<\/p>\n<\/p><\/div>\n<\/section>\n

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Training and Scheduling Use<\/h2>\n<\/header>\n

Contact the Research Supervisor to arrange training. Training is for instrument use only. Please do not bring experimental samples to training without prior approval.<\/p>\n

Use of the CORE facility is primarily for trained users although the Research Supervisor may be requested to operate the systems. Schedule planned usage on the calendars located by each piece of equipment.<\/p>\n<\/p><\/div>\n<\/section>\n

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Rates (Effective January 1, 2019)<\/h2>\n<\/header>\n\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t
Equipment<\/th>WSU<\/th>Non-WSU<\/th>\n<\/tr>\n<\/thead>\n
Galios<\/td>$45\/hr<\/td>$50\/hr<\/td>\n<\/tr>\n
Accuri<\/td>$40\/hr<\/td>$45\/hr<\/td>\n<\/tr>\n
S3 Cell Sorter<\/td>$100\/hr<\/td>$110\/hr<\/td>\n<\/tr>\n
Computer Use Only<\/td>$5\/hr<\/td>$6\/hr<\/td>\n<\/tr>\n
Technical Support<\/td>$45\/hr<\/td>$50\/hr<\/td>\n<\/tr>\n
Training<\/td>$45\/hr<\/td>$50\/hr<\/td>\n<\/tr>\n
Misuse Fee<\/td>To Cover Cost of Cleaning\/Repair<\/td>To Cover Cost of Cleaning\/Repair<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n<\/p><\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"

Flow Cytometry<\/p>\n

Flow Cytometry is a highly quantitative high throughput technique to accurately detect and quantitate various cell components at the single cell level.<\/p>\n

Contact Us<\/p>\n

Ze Liu<\/strong>
Research Supervisor
509-358-7633
ze.liu@wsu.edu<\/a><\/p>\n

Bio-Rad S3 Cell Sorter<\/h2>\n

This automated benchtop cell sorter is equipped with 2 lasers, 4 fluorescense detectors, and forward- and side-scatter detectors. Features of the unit include simplified instrument setup, reduced carryover, 2-way cell sorting, high speed and high purity, and intuitive interface.<\/p>\n

Beckman Coulter Gallios<\/h2>\n

This unit is a research system that delivers analytical excellence by coupling extraordinary sensitivity resolution and dynamic range with high … » More …<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-builder.php","meta":[],"wsuwp_university_location":[],"wsuwp_university_org":[],"_links":{"self":[{"href":"https:\/\/labs.wsu.edu\/flowcore\/wp-json\/wp\/v2\/pages\/2"}],"collection":[{"href":"https:\/\/labs.wsu.edu\/flowcore\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/labs.wsu.edu\/flowcore\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/flowcore\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/flowcore\/wp-json\/wp\/v2\/comments?post=2"}],"version-history":[{"count":9,"href":"https:\/\/labs.wsu.edu\/flowcore\/wp-json\/wp\/v2\/pages\/2\/revisions"}],"predecessor-version":[{"id":37,"href":"https:\/\/labs.wsu.edu\/flowcore\/wp-json\/wp\/v2\/pages\/2\/revisions\/37"}],"wp:attachment":[{"href":"https:\/\/labs.wsu.edu\/flowcore\/wp-json\/wp\/v2\/media?parent=2"}],"wp:term":[{"taxonomy":"wsuwp_university_location","embeddable":true,"href":"https:\/\/labs.wsu.edu\/flowcore\/wp-json\/wp\/v2\/wsuwp_university_location?post=2"},{"taxonomy":"wsuwp_university_org","embeddable":true,"href":"https:\/\/labs.wsu.edu\/flowcore\/wp-json\/wp\/v2\/wsuwp_university_org?post=2"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}