Illumina Sequencing
Workflow
Workflow of Typical Sequencing Projects Performed at the Core
Pricing Information and Options
Library Preparation
(all prices are per sample)
| Application Type | Examples of Reagents/Kits | Price |
|---|---|---|
| RNA-seq Library/mRNA | TruSeq RNA Prep v2 | $168 |
| RNA-seq Library/Total RNA | TruSeq Stranded Total with Ribo-zero | $221 |
| small RNA Library | TruSeq small RNA | $207 |
| Low Input RNA (10 pg) Library | SMARTer | $290 |
| ChIP-seq Library | TruSeq ChIP | $184 |
| Whole Genome Library | TruSeq Nano | $141 |
| Exome Library | Nextera Rapid Capture Exome | $256 |
| Methylation Library | EpiGome Methyl-Seq | $200 |
| Moleculo Library | Truseq Synthetic Long-Read DNA Library Prep Kit; TruSeq Synthetic Long-Read DNA Barcode Kit | $1,828 |
| Amplicon-based Library | Please inquire | Please inquire |
| De Novo Sequencing Library | Please inquire | Please inquire |
| Other Libraries | Please inquire | Please inquire |
Quant/QC of Libraries and Nucleic Acids
| Application Type | Reagents | Price |
|---|---|---|
| Library Quant by qPCR (per sample) | Kapa Library Quantification Kit | $30 |
| DNA/RNA Quant Qubit (per sample) | Qubit Assay Reagents | $5 |
| Library QC (per sample) | Fragment Analyzer Reagents | $10 |
Illumina Novaseq Sequencing
| Novaseq | Unit | Price |
|---|---|---|
| 1 billion reads per lane | Lane | $2,540 |
| Please inquire about other sequencing options |
Illumina Hiseq Sequencing
| Hiseq | Unit | Price |
|---|---|---|
| 380 million reads per lane | Lane | $2,140 |
| Please inquire about other sequencing options |
Illumina NextSeq Sequencing
| High Output v2 kit 400 million reads per run | Unit | Price |
|---|---|---|
| Single Read 1x75 bp | Run | $2,039 |
| Single Read 1x150 bp | Run | $3,939 |
| Paired-End 2x75 bp | Run | $3,947 |
| Paired-End 2x150 bp | Run | $6,306 |
| Mid Output v2 kit 130 million reads per run | Unit | Price |
| Single Read 1x150 bp | Run | $1,489 |
| Pair-End Read 2x75 bp | Run | $1,499 |
| Pair-End Read 2x150 bp | Run | $2,389 |