{"id":249,"date":"2021-03-30T17:26:28","date_gmt":"2021-03-31T00:26:28","guid":{"rendered":"https:\/\/labs.wsu.edu\/johnclarke\/?page_id=249"},"modified":"2021-03-31T16:31:47","modified_gmt":"2021-03-31T23:31:47","slug":"microcystin-lr-hepatotoxicity-in-nafld","status":"publish","type":"page","link":"https:\/\/labs.wsu.edu\/johnclarke\/toxicities-in-nafld\/microcystin-lr-hepatotoxicity-in-nafld\/","title":{"rendered":"Microcystin-LR hepatotoxicity"},"content":{"rendered":"<br \/>\n<section id=\"builder-section-1617209188342\" class=\"row single gutter pad-top\">\n<div style=\"\" class=\"column one \">\n<header>\n<h2>Reduced hepatic recovery after MCLR toxicity in NASH<\/h2>\n<\/header>\n<p style=\"text-align: left\">Nonalcoholic steatohepatitis (NASH) causes liver extracellular matrix (ECM) remodeling and is a<br \/>\nrisk factor for fibrosis and hepatocellular carcinoma (HCC). Microcystin-LR (MCLR) is a<br \/>\nhepatotoxin produced by fresh-water cyanobacteria that causes a NASH-like phenotype, liver<br \/>\nfibrosis, and is a risk factor for HCC. The current study investigated hepatic recovery after MCLR elicited<br \/>\nliver injury in pre-existing NASH. Rats were fed either a control or a high fat\/high<br \/>\ncholesterol (HFHC) diet for eight weeks. Animals received either vehicle or 30 \u03bcg\/kg MCLR (i.p:<br \/>\n2 weeks, alternate days). Animals were euthanized at one of three time points: at the completion<br \/>\nof the MCLR exposure period and after 2 and 4 weeks of recovery. Histological staining suggested<br \/>\nthat after four weeks of recovery the MCLR-exposed HFHC group had less steatosis and more<br \/>\nfibrosis compared to the vehicle-exposed HFHC group and MCLR-exposed control group. RNA-Seq<br \/>\nanalysis revealed dysregulation of ECM genes after MCLR exposure in both control and<br \/>\nHFHC groups that persisted only in the HFHC groups during recovery. After 4 weeks of recovery,<br \/>\nMCLR hepatotoxicity in pre-existing NASH persistently dysregulated genes related to cellular<br \/>\ndifferentiation and HCC. These data demonstrate impaired hepatic recovery and persistent<br \/>\ncarcinogenic changes after MCLR toxicity in pre-existing NASH.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-258\" src=\"https:\/\/wpcdn.web.wsu.edu\/wp-labs\/uploads\/sites\/1669\/2021\/03\/Graphical-abstract-396x195.jpg\" alt=\"\" width=\"593\" height=\"292\" srcset=\"https:\/\/wpcdn.web.wsu.edu\/wp-labs\/uploads\/sites\/1669\/2021\/03\/Graphical-abstract-396x195.jpg 396w, https:\/\/wpcdn.web.wsu.edu\/wp-labs\/uploads\/sites\/1669\/2021\/03\/Graphical-abstract-792x391.jpg 792w, https:\/\/wpcdn.web.wsu.edu\/wp-labs\/uploads\/sites\/1669\/2021\/03\/Graphical-abstract-768x379.jpg 768w, https:\/\/wpcdn.web.wsu.edu\/wp-labs\/uploads\/sites\/1669\/2021\/03\/Graphical-abstract-990x489.jpg 990w, https:\/\/wpcdn.web.wsu.edu\/wp-labs\/uploads\/sites\/1669\/2021\/03\/Graphical-abstract.jpg 1149w\" sizes=\"(max-width: 593px) 100vw, 593px\" \/><\/p>\n<\/p><\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p> <\/p>\n<h2>Reduced hepatic recovery after MCLR toxicity in NASH<\/h2>\n<p style=\"text-align: left\">Nonalcoholic steatohepatitis (NASH) causes liver extracellular matrix (ECM) remodeling and is a<br \/> risk factor for fibrosis and hepatocellular carcinoma (HCC). Microcystin-LR (MCLR) is a<br \/> hepatotoxin produced by fresh-water cyanobacteria that causes a NASH-like phenotype, liver<br \/> fibrosis, and is a risk factor for HCC. The current study investigated hepatic recovery after MCLR elicited<br \/> liver injury in pre-existing NASH. Rats were fed either a control or a high fat\/high<br \/> cholesterol (HFHC) diet for eight weeks. Animals received either vehicle or 30 \u03bcg\/kg MCLR (i.p:<br \/> 2 weeks, alternate days). Animals &#8230; <a href=\"https:\/\/labs.wsu.edu\/johnclarke\/toxicities-in-nafld\/microcystin-lr-hepatotoxicity-in-nafld\/\" class=\"more-link\"><span class=\"more-default\">&raquo; More &#8230;<\/span><\/a><\/p>\n","protected":false},"author":4864,"featured_media":0,"parent":62,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-builder.php","meta":[],"wsuwp_university_location":[],"wsuwp_university_org":[],"_links":{"self":[{"href":"https:\/\/labs.wsu.edu\/johnclarke\/wp-json\/wp\/v2\/pages\/249"}],"collection":[{"href":"https:\/\/labs.wsu.edu\/johnclarke\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/labs.wsu.edu\/johnclarke\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/johnclarke\/wp-json\/wp\/v2\/users\/4864"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/johnclarke\/wp-json\/wp\/v2\/comments?post=249"}],"version-history":[{"count":4,"href":"https:\/\/labs.wsu.edu\/johnclarke\/wp-json\/wp\/v2\/pages\/249\/revisions"}],"predecessor-version":[{"id":283,"href":"https:\/\/labs.wsu.edu\/johnclarke\/wp-json\/wp\/v2\/pages\/249\/revisions\/283"}],"up":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/johnclarke\/wp-json\/wp\/v2\/pages\/62"}],"wp:attachment":[{"href":"https:\/\/labs.wsu.edu\/johnclarke\/wp-json\/wp\/v2\/media?parent=249"}],"wp:term":[{"taxonomy":"wsuwp_university_location","embeddable":true,"href":"https:\/\/labs.wsu.edu\/johnclarke\/wp-json\/wp\/v2\/wsuwp_university_location?post=249"},{"taxonomy":"wsuwp_university_org","embeddable":true,"href":"https:\/\/labs.wsu.edu\/johnclarke\/wp-json\/wp\/v2\/wsuwp_university_org?post=249"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}