Sleep and Performance Research Center
Molecular And Genetic Approaches to Sleep
Jonathan P. Wisor, Ph.D.
AREAS OF INTEREST
Drugs of Abuse and Sleep, Sleep Regulatory Neuronal Populations, Cerebral Metabolic Changes with Sleep
Humans spend roughly one-third of their lives asleep. Sleep insufficiency has a number of negative effects on health and well-being. The function that is served by sleep and the mechanisms by which sleep insufficiency causes health problems are not well understood. The purpose of my research program is to identify functional consequences of sleep and sleep loss within the nervous system.
My laboratory has received funding from the Department of Defense (Defense Advanced Research Projects Agency, Young Faculty Award) and the National Institute of Neurological Disorders and Stroke to apply molecular genetic and biochemical techniques in studies of the regulation of sleep.
Sleep regulatory cell populations and molecules
Our projects are aimed at characterizing the roles of genetic loci and discrete cell populations within the cerebral cortex in regulating sleep and sleep-related electroencephalographic wave forms. We use gene expression and protein profiling in neuronal and non-neuronal brain cell populations to identify putative regulators of sleep. We use germ line targeted optogenetic constructs to modulate the activity of discrete cell types and measure the effects of these manipulations on sleep. Working with research partners, we perform studies on human subject populations to apply the findings of basic neurobiological studies to issues related to human health.
STAFF AND TRAINEES
Will Clegern BS (research technologist)
Michelle Schmidt MS (research technologist)
Janne Grønli PhD (research assistant professor)
Michael Rempe PhD (adjunct professor)
Cerebral metabolic monitoring
Gene expression analysis (qPCR, RNAseq)
Time/frequency analysis of EEG waveforms
- NATIONAL INSTITUTES OF HEALTH:
- DEFENSE ADVANCED RESEARCH PROJECTS AGENCY