{"id":2474,"date":"2019-10-23T12:10:56","date_gmt":"2019-10-23T19:10:56","guid":{"rendered":"https:\/\/labs.wsu.edu\/tanaka-lab\/?p=2474"},"modified":"2019-11-21T08:50:08","modified_gmt":"2019-11-21T16:50:08","slug":"flgii-28-potato-mpmi","status":"publish","type":"post","link":"https:\/\/labs.wsu.edu\/tanaka-lab\/2019\/10\/23\/flgii-28-potato-mpmi\/","title":{"rendered":"Our article about FlgII-28-induced defense responses in potato was published in MPMI"},"content":{"rendered":"<p><strong>October 23, 2019<\/strong> \u2013 Our article entitled \u201cFlgII-28 is a Major Flagellin-Derived Defense Elicitor in Potato\u201d was published in <em>Molecular Plant-Microbe Interactions<\/em>.\u00a0<!--more--><\/p>\n<p><strong>How to cite:<br \/>\n<\/strong>Moroz N, Tanaka K (2020) FlgII-28 is a major flagellin-derived defense elicitor for potato. Molecular Plant-Microbe Interactions. <a href=\"https:\/\/doi.org\/10.1094\/MPMI-06-19-0164-R\">doi:10.1094\/MPMI-06-19-0164-R<\/a><\/p>\n<p><strong>Abstract:<\/strong><br \/>\nThe first layer of plant immunity is deployed by recognition of pathogen-associated molecule patterns (PAMPs) and induction of early stress responses. Flagellin is the major protein component of the flagellum. Flagellin-derived peptide fragments, such as Flg22, a short active peptide derived from the highly conserved part of the N-terminal region, are recognized as PAMPs by a specific perception system present in most higher plants. Some bacteria evade the plant recognition system by altering the Flg22 region in the flagellin. Instead, a small subset of plants (i.e., Solanaceous plants) can sense these bacteria by recognizing a second region, termed FlgII-28. The function of FlgII-28 has been well documented in tomato but not in potato plants. Here, we investigated the effect of FlgII-28 on several defense responses in potato. Cytosolic calcium (Ca<sup>2+<\/sup>) elevation is an early defense response upon pathogenic infection. We generated transgenic potato plants expressing aequorin, a nontoxic Ca<sup>2+<\/sup>-activated photoprotein. The results showed that FlgII-28 stimulated strong cytosolic Ca<sup>2+<\/sup> elevation in a dose-dependent manner, whereas the response was attenuated when a Ca<sup>2+<\/sup> channel blocker was added. In addition, the FlgII-28-stimulated cytosolic Ca<sup>2+<\/sup> elevation was shown to subsequently promote extracellular alkalinization, reactive oxygen species production, mitogen-activated protein kinase phosphorylation, and transcriptional reprogramming of defense-related genes in potato. Interestingly, all tested defense responses caused by FlgII-28 were significantly stronger than those caused by Flg22, suggesting that FlgII-28 acts as a primary flagellar PAMP to elicit multiple defense responses in potato.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p><strong>October 23, 2019<\/strong> \u2013 Our article entitled \u201cFlgII-28 is a Major Flagellin-Derived Defense Elicitor in Potato\u201d was published in <em>Molecular Plant-Microbe Interactions<\/em>.\u00a0<\/p>\n","protected":false},"author":2755,"featured_media":2480,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[],"tags":[],"wsuwp_university_location":[],"wsuwp_university_org":[],"_links":{"self":[{"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/posts\/2474"}],"collection":[{"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/users\/2755"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/comments?post=2474"}],"version-history":[{"count":5,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/posts\/2474\/revisions"}],"predecessor-version":[{"id":2498,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/posts\/2474\/revisions\/2498"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/media\/2480"}],"wp:attachment":[{"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/media?parent=2474"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/categories?post=2474"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/tags?post=2474"},{"taxonomy":"wsuwp_university_location","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/wsuwp_university_location?post=2474"},{"taxonomy":"wsuwp_university_org","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/wsuwp_university_org?post=2474"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}