{"id":2629,"date":"2020-09-25T07:10:48","date_gmt":"2020-09-25T14:10:48","guid":{"rendered":"https:\/\/labs.wsu.edu\/tanaka-lab\/?p=2629"},"modified":"2020-09-25T09:02:51","modified_gmt":"2020-09-25T16:02:51","slug":"purinoceptor-plant-resistance-root-pathogen","status":"publish","type":"post","link":"https:\/\/labs.wsu.edu\/tanaka-lab\/2020\/09\/25\/purinoceptor-plant-resistance-root-pathogen\/","title":{"rendered":"We published a new article regarding the purinocepter P2K1 as a novel resistance component to a root fungal pathogen"},"content":{"rendered":"<p><strong>September 25, 2020<\/strong>\u00a0\u2013 Our research article entitled \u201cPurinoceptor P2K1\/DORN1 enhances plant resistance against a soilborne fungal pathogen,\u00a0<em>Rhizoctonia solani<\/em>\u201d was published in <a href=\"https:\/\/doi.org\/10.3389\/fpls.2020.572920\"><em>Frontiers in Plant Science<\/em><\/a>.\u00a0<!--more--><\/p>\n<p><strong>How to cite:<br \/>\n<\/strong>Kumar S,\u00a0Tripathi D, Okubara PA,\u00a0Tanaka K\u00a0(2020) Purinoceptor P2K1\/DORN1 enhances plant resistance against a soilborne fungal pathogen,\u00a0<em>Rhizoctonia solani<\/em>.\u00a0<em>Front. Plant Sci.<\/em> 11: 572920. <a href=\"https:\/\/doi.org\/10.3389\/fpls.2020.572920\">doi:10.3389\/fpls.2020.572920<\/a><\/p>\n<p><strong>Abstract:<\/strong><br \/>\nThe purinoceptor P2K1\/DORN1 recognizes extracellular ATP, a damage-associated molecular pattern (DAMP) released upon cellular disruption by wounding and necrosis, which in turn, boost plant innate immunity. P2K1 is known to confer plant resistance to foliar biotrophic, hemi-biotrophic, and necrotrophic pathogens. However, until now, no information was available on its activity against root pathogens. In this report, we describe the contribution of P2K1 to resistance in Arabidopsis against <em>Rhizoctonia solani<\/em>, a broad host range, necrotrophic soilborne fungal pathogen. In pot assays, the Arabidopsis P2K1 overexpression line <em>OxP2K<\/em>1 showed longer root length and a greater rosette surface area than wild type in the presence of the pathogen. In contrast, the knockout mutant <em>dorn1-3<\/em> and the double mutant <em>rbohd\/f<\/em>, defective in two subunits of the respiratory burst complex NADPH oxidase, exhibited significant reductions in shoot and root lengths and rosette surface area compared to wild type when the pathogen was present. Root expression of <em>PR1<\/em>, <em>PDF1.2<\/em> and <em>JAZ5<\/em> was reduced in <em>dorn1-3<\/em> and <em>rbohd\/f<\/em> and elevated in <em>OxP2K1<\/em> relative to wild type, indicating that the salicylate and jasmonate defense signaling pathways functioned in resistance. These results indicated that a DAMP-mediated defense system confers basal resistance against an important root necrotrophic fungal pathogen.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p><strong>September 25, 2020<\/strong>\u00a0\u2013 Our research article entitled \u201cPurinoceptor P2K1\/DORN1 enhances plant resistance against a soilborne fungal pathogen,\u00a0<em>Rhizoctonia solani<\/em>\u201d was published in <a href=\"https:\/\/doi.org\/10.3389\/fpls.2020.572920\"><em>Frontiers in Plant Science<\/em><\/a>.\u00a0<\/p>\n","protected":false},"author":2755,"featured_media":2627,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[],"tags":[],"wsuwp_university_location":[],"wsuwp_university_org":[],"_links":{"self":[{"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/posts\/2629"}],"collection":[{"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/users\/2755"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/comments?post=2629"}],"version-history":[{"count":11,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/posts\/2629\/revisions"}],"predecessor-version":[{"id":2645,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/posts\/2629\/revisions\/2645"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/media\/2627"}],"wp:attachment":[{"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/media?parent=2629"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/categories?post=2629"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/tags?post=2629"},{"taxonomy":"wsuwp_university_location","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/wsuwp_university_location?post=2629"},{"taxonomy":"wsuwp_university_org","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/wsuwp_university_org?post=2629"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}