{"id":3309,"date":"2023-06-02T17:05:58","date_gmt":"2023-06-03T00:05:58","guid":{"rendered":"https:\/\/labs.wsu.edu\/tanaka-lab\/?p=3309"},"modified":"2023-08-08T17:19:41","modified_gmt":"2023-08-09T00:19:41","slug":"eatp-gus-reporter-frontiers","status":"publish","type":"post","link":"https:\/\/labs.wsu.edu\/tanaka-lab\/2023\/06\/02\/eatp-gus-reporter-frontiers\/","title":{"rendered":"Histochemical reporter system tracks plant root&#8217;s eATP response, published in Frontiers Journal"},"content":{"rendered":"<p><strong>June 2, 2023<\/strong> \u2013 A spatiotemporal monitoring tool of eATP response in the plant root tip was published in <em><a href=\"https:\/\/doi.org\/10.3389\/fpls.2023.1183335\">Frontiers in Plant Sciences<\/a><\/em>. Congrats to Joel on his 1st first-authored paper!\u00a0 <!--more--><\/p>\n<p><strong>How to cite:<\/strong><strong><br \/>\n<\/strong>Sowders JM,\u00a0Tanaka K\u00a0(2023) A histochemical reporter system to study extracellular ATP response in plants.\u00a0<em>Frontiers in Plant Sciences<\/em>\u00a014: 1183335\u00a0<a href=\"https:\/\/doi.org\/10.3389\/fpls.2023.1183335\">https:\/\/doi.org\/10.3389\/fpls.2023.1183335<\/a><\/p>\n<p><strong>Abstract:<br \/>\n<\/strong><\/p>\n<div class=\"JournalAbstract\">\n<p>When cells experience acute mechanical distress, they release ATP from their cellular compartment into the surrounding microenvironment. This extracellular ATP (eATP) can then act as a danger signal\u2014signaling cellular damage. In plants, cells adjacent to damage detect rising eATP concentrations through the cell-surface receptor kinase, P2K1. Following eATP perception, P2K1 initiates a signaling cascade mobilizing plant defense. Recent transcriptome analysis revealed a profile of eATP-induced genes sharing pathogen- and wound-response hallmarks\u2014consistent with a working model for eATP as a defense-mobilizing danger signal. To build on the transcriptional footprint and broaden our understanding of dynamic eATP signaling responses in plants, we aimed to i) generate a visual toolkit for eATP-inducible marker genes using a \u03b2-glucuronidase (GUS) reporter system and ii) evaluate the spatiotemporal response of these genes to eATP in plant tissues. Here, we demonstrate that the promoter activities of five genes, <i>ATPR1<\/i>,\u00a0<i>ATPR2<\/i>,\u00a0<i>TAT3<\/i>,\u00a0<i>WRKY46<\/i>, and\u00a0<i>CNGC19<\/i>, were highly sensitive to eATP in the primary root meristem and elongation zones with maximal responses at 2 h after treatment. These results suggest the primary root tip as a hub to study eATP-signaling activity and provide a proof-of-concept toward using these reporters to further dissect eATP and damage signaling in plants.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p><strong>June 2, 2023<\/strong> \u2013 A spatiotemporal monitoring tool of eATP response in the plant root tip was published in <em><a href=\"https:\/\/doi.org\/10.3389\/fpls.2023.1183335\">Frontiers in Plant Sciences<\/a><\/em>. Congrats to Joel on his 1st first-authored paper!\u00a0 <\/p>\n","protected":false},"author":2755,"featured_media":3272,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[],"tags":[],"wsuwp_university_location":[],"wsuwp_university_org":[],"_links":{"self":[{"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/posts\/3309"}],"collection":[{"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/users\/2755"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/comments?post=3309"}],"version-history":[{"count":3,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/posts\/3309\/revisions"}],"predecessor-version":[{"id":3316,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/posts\/3309\/revisions\/3316"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/media\/3272"}],"wp:attachment":[{"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/media?parent=3309"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/categories?post=3309"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/tags?post=3309"},{"taxonomy":"wsuwp_university_location","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/wsuwp_university_location?post=3309"},{"taxonomy":"wsuwp_university_org","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/wsuwp_university_org?post=3309"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}