{"id":947,"date":"2017-01-24T00:01:34","date_gmt":"2017-01-24T08:01:34","guid":{"rendered":"https:\/\/labs.wsu.edu\/tanaka-lab\/?p=947"},"modified":"2018-08-23T13:26:14","modified_gmt":"2018-08-23T20:26:14","slug":"january-2017","status":"publish","type":"post","link":"https:\/\/labs.wsu.edu\/tanaka-lab\/2017\/01\/24\/january-2017\/","title":{"rendered":"Our new article &#8220;Extracellular Alkalinization as a Defense Response in Potato Cells&#8221; published in a Frontiers journal"},"content":{"rendered":"<p><strong>January 24, 2017<\/strong> \u2013 Our new article entitled\u00a0&#8220;<a href=\"http:\/\/dx.doi.org\/10.3389\/fpls.2017.00032\">Extracellular alkalinization as a defense response in potato cells<\/a>&#8221; was published in <em>Frontiers in Plant Science<\/em>.\u00a0<!--more-->We congratulate Moroz\u00a0<em>et al.<\/em> on the\u00a0publication in a good team setting.<\/p>\n<h6>Abstract<\/h6>\n<p>A quantitative and robust bioassay to assess plant defense response is important for studies of disease resistance and also for the early identification of disease during pre- or non-symptomatic phases. An increase in extracellular pH is known to be an early defense response in plants. In this study, we demonstrate extracellular alkalinization as a defense response in potatoes. Using potato suspension cell cultures, we observed an alkalinization response against various pathogen- and plant-derived elicitors in a dose- and time-dependent manner. We also assessed the defense response against a variety of potato pathogens, such as protists (<i>Phytophthora infestans<\/i> and <i>Spongospora subterranea<\/i>) and fungi (<i>Verticillium dahliae<\/i> and <i>Colletotrichum coccodes<\/i>). Our results show that extracellular pH increases within 30 min in proportion to the number of pathogen spores added. Consistently with the alkalinization effect, the higher transcription level of several defense-related genes and production of reactive oxygen species was observed. Our results demonstrate that the alkalinization response is an effective marker to study early stages of defense response in potatoes.<\/p>\n<p>Read full text of the publication\u00a0<span style=\"text-decoration: underline\"><a href=\"http:\/\/dx.doi.org\/10.3389\/fpls.2017.00032\">here<\/a><\/span>.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p><strong>January 24, 2017<\/strong> \u2013 Our new article entitled\u00a0&#8220;<a href=\"http:\/\/dx.doi.org\/10.3389\/fpls.2017.00032\">Extracellular alkalinization as a defense response in potato cells<\/a>&#8221; was published in <em>Frontiers in Plant Science<\/em>.\u00a0<\/p>\n","protected":false},"author":2755,"featured_media":950,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[],"tags":[],"wsuwp_university_location":[],"wsuwp_university_org":[],"_links":{"self":[{"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/posts\/947"}],"collection":[{"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/users\/2755"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/comments?post=947"}],"version-history":[{"count":21,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/posts\/947\/revisions"}],"predecessor-version":[{"id":952,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/posts\/947\/revisions\/952"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/media\/950"}],"wp:attachment":[{"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/media?parent=947"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/categories?post=947"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/tags?post=947"},{"taxonomy":"wsuwp_university_location","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/wsuwp_university_location?post=947"},{"taxonomy":"wsuwp_university_org","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tanaka-lab\/wp-json\/wp\/v2\/wsuwp_university_org?post=947"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}