{"id":570,"date":"2021-10-28T15:20:14","date_gmt":"2021-10-28T22:20:14","guid":{"rendered":"http:\/\/labs.wsu.edu\/tipl\/?page_id=570"},"modified":"2021-10-28T15:35:41","modified_gmt":"2021-10-28T22:35:41","slug":"cutting-gels","status":"publish","type":"page","link":"https:\/\/labs.wsu.edu\/tipl\/tipl-resource-page\/in-gel-digestion-samples\/cutting-gels\/","title":{"rendered":"Cutting gels"},"content":{"rendered":"<br \/>\n<section id=\"builder-section-1635460119053\" class=\"row single gutter pad-top\">\n<div style=\"\" class=\"column one \">\n<header>\n<h2>Cutting gels<\/h2>\n<\/header>\n<p>If you are going to cut out the gel bands yourself from a gel, please follow carefully the following instructions:<\/p>\n<ul>\n<li>Clean the gel box or surface you are cutting the gel on\/in with\u00a0ethanol, isopropanol or methanol before the gel comes in contact with it.<\/li>\n<li>Use a clean razor\/scalpel blade, and clean it further with ethanol, isopropanol or methanol before cutting.<\/li>\n<\/ul>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"wp-image-574 alignnone\" src=\"https:\/\/wpcdn.web.wsu.edu\/wp-labs\/uploads\/sites\/629\/2021\/10\/Excel-Hobby-Blades-Corp_G1415728xx1xxe14496-198x198.jpeg\" alt=\"\" width=\"144\" height=\"144\" srcset=\"https:\/\/wpcdn.web.wsu.edu\/wp-labs\/uploads\/sites\/629\/2021\/10\/Excel-Hobby-Blades-Corp_G1415728xx1xxe14496-198x198.jpeg 198w, https:\/\/wpcdn.web.wsu.edu\/wp-labs\/uploads\/sites\/629\/2021\/10\/Excel-Hobby-Blades-Corp_G1415728xx1xxe14496-400x400.jpeg 400w, https:\/\/wpcdn.web.wsu.edu\/wp-labs\/uploads\/sites\/629\/2021\/10\/Excel-Hobby-Blades-Corp_G1415728xx1xxe14496-396x396.jpeg 396w, https:\/\/wpcdn.web.wsu.edu\/wp-labs\/uploads\/sites\/629\/2021\/10\/Excel-Hobby-Blades-Corp_G1415728xx1xxe14496.jpeg 450w\" sizes=\"(max-width: 144px) 100vw, 144px\" \/><\/p>\n<ul>\n<li>Handle and cut the gel in a laminar flow clean hood if one is available in your lab.<\/li>\n<li>Cut out a maximum of 10mm of a single gel lane for each sample you will be submitting -but-<\/li>\n<li>Don\u2019t cut out unstained gel surrounding a band \u2013 cut tightly to the stained area.<\/li>\n<li>Slice the cut gel band into 1mm cubes, but no smaller.<\/li>\n<li>Place the sliced band into a high-quality Eppendorf microcentrifuge tube, which has been rinsed with 50% organic solvent and millipure water.<\/li>\n<li>Close the tube as soon as your bands are inside!<\/li>\n<li><\/li>\n<\/ul><\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p> <\/p>\n<h2>Cutting gels<\/h2>\n<p>If you are going to cut out the gel bands yourself from a gel, please follow carefully the following instructions:<\/p>\n<p>Clean the gel box or surface &#8230; <a href=\"https:\/\/labs.wsu.edu\/tipl\/tipl-resource-page\/in-gel-digestion-samples\/cutting-gels\/\" class=\"more-link\"><span class=\"more-default\">&raquo; More &#8230;<\/span><\/a><\/p>\n","protected":false},"author":20665,"featured_media":555,"parent":552,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-builder.php","meta":[],"wsuwp_university_location":[],"wsuwp_university_org":[],"_links":{"self":[{"href":"https:\/\/labs.wsu.edu\/tipl\/wp-json\/wp\/v2\/pages\/570"}],"collection":[{"href":"https:\/\/labs.wsu.edu\/tipl\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/labs.wsu.edu\/tipl\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/tipl\/wp-json\/wp\/v2\/users\/20665"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/tipl\/wp-json\/wp\/v2\/comments?post=570"}],"version-history":[{"count":7,"href":"https:\/\/labs.wsu.edu\/tipl\/wp-json\/wp\/v2\/pages\/570\/revisions"}],"predecessor-version":[{"id":576,"href":"https:\/\/labs.wsu.edu\/tipl\/wp-json\/wp\/v2\/pages\/570\/revisions\/576"}],"up":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/tipl\/wp-json\/wp\/v2\/pages\/552"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/labs.wsu.edu\/tipl\/wp-json\/wp\/v2\/media\/555"}],"wp:attachment":[{"href":"https:\/\/labs.wsu.edu\/tipl\/wp-json\/wp\/v2\/media?parent=570"}],"wp:term":[{"taxonomy":"wsuwp_university_location","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tipl\/wp-json\/wp\/v2\/wsuwp_university_location?post=570"},{"taxonomy":"wsuwp_university_org","embeddable":true,"href":"https:\/\/labs.wsu.edu\/tipl\/wp-json\/wp\/v2\/wsuwp_university_org?post=570"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}