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WSU Spokane Flow Cytometry Core

Flow cytometry is a highly quantitative high throughput technique to accurately detect and quantitate various cell components at the single cell level. The Flow Cytometry core facility is located on the third floor of the PBS building and is equipped with the necessary instrumentation for your cell analysis and sorting needs.

Cytometers

CytometerExcitation LaserAcquisition Channel/FilterExample Fluorescent ProteinsAdditional Features
Bio-rad S3 Cell Sorter488nm
561nm
Co-linear
Fl1- 525/30
Fl2- 586/25
Fl3- 615/25
Fl4- 655LP
FitC, AF488, GFP
PE
PE-TR, PI
mCherry from 561nm
PerCP, PE-cy7
Sorter - 10,000 events/sec+. 561nm laser for better detection of mCherry, mStrawberry, etc.
Beckman Coulter Gallios488nm
638nm
Fl1- 525/40
Fl2- 575/30
Fl3- 620/30
Fl4- 695/30
Fl5- 755LP
Fl6- 660/20
FitC, AF488, GFP
PE
PE-TR, PI
PerCP, PE-Cy5.5
PE-Cy7, PE-AF700
APC, AF647
Thirty-two sample automated carrousel, and extra carrousel for quick swap. Seven logs of fluorescence detection. Kaluza acquisition and analysis software.
Six color analysis
BD Accuri C6488nm
640nm
Fl1- 510/15
530/30
Fl2- 585/40
540/20
Fl3- 670LP
610/20
Fl4- 675/25
GFP
FitC, AF488
PE, PI
YFP (bleeds into 530)
PerCP, PE-cy5.5
PI, PE-TR
APC, AF647, AF700
Simultaneous detection of GFP and YFP. Easy change filters. Csampler arm attachment for analysis of 24 1.5 mL tubes to 96 well plates. Set laser voltage- great for beginners. C6acquisition software with analysis capabilities.

Pricing

Pricing structure is on a per-hour basis, and differs depending on the machine used:

 

EquipmentInternal RateExternal Rate
BD Accuri C6$14 / hour$15 / hour
Beckman Coulter Gallios$40 / hour$43 / hour
Bio-Rad S3 Cell Sorter$50 / hour$54 / hour
Training$45 / hour$49 / hour

Software

Each cytometer has its own acquisition software, specifically designed to optimally run the machine and acquire your data. All files can be saved as general format FCS files and further analyzed with Beckman Coulter Kaluza analysis software available for use in the Core.

Training and Scheduling Use

Use of the core facility is primarily for unassisted acquisition and analysis following appropriate training. Please contact the Joseph Cook to schedule training* and flow experiment consultation.

*Training will be for instrument use only. Please do not bring experimental samples to training without prior approval.