Flow Cytometry
Flow Cytometry is a highly quantitative high throughput technique to accurately detect and quantitate various cell components at the single cell level.
Contact Us
Ze Liu
Research Supervisor
509-358-7633
ze.liu@wsu.edu
Bio-Rad S3 Cell Sorter
This automated benchtop cell sorter is equipped with 2 lasers, 4 fluorescense detectors, and forward- and side-scatter detectors. Features of the unit include simplified instrument setup, reduced carryover, 2-way cell sorting, high speed and high purity, and intuitive interface.
Excitation Laser | Acquisition Channel/Filter | Example Fluorescent Proteins |
---|---|---|
488 nm 561 nm Co-linear | FI1 – 525/30 FI2 – 586/25 FI3 – 615/25 FI4 – 655LP | FitC, AF488, GFP, PE, PE-TR, PI, mCherry from 561nm, PerCEP, PE-cy7 |
Beckman Coulter Gallios
This unit is a research system that delivers analytical excellence by coupling extraordinary sensitivity resolution and dynamic range with high speed data collection. Along with excellent detection capabilities, the Gallios includes easy to use software and automation to facilitate superior performance of multi-color flow cytometry assays.
Excitation Laser | Acquisition Channel/Filter | Example Fluorescent Proteins |
---|---|---|
488 nm 638 nm | FI1 – 525/40 FI2 – 575/30 FI3 – 620/30 FI4 – 695/30 FI5 – 755LP FI6 – 660/20 | FitC, AF488, GFP, PE, PE-TR, PI, PerCP, PE-Cy5.5, PE-Cy7, PE-AF700, APC, AF647 |
Becton Dickinson Accuri C6
This unit is equipped with 2 lasers, 2 light scatter detectors, and 4 fluroescense detectors with optical filters optimized for the detrection pf FITC, PE, PerCP, APC. Data is digitally collected over a wide dynamic range of 7.2 decades, making all data available to users as needed.
Excitation Laser | Acquisition Channel/Filter | Example Fluorescent Proteins |
---|---|---|
488 nm 640 nm | FI1 – 510/15 530/30 FI2 – 585/40 540/20 FI3 – 670LP 610/20 FI4 – 675/25 | GFP, FitC, AF488, PE, PI, YFP (bleeds into 530), PerCP, PE-cy5.5, PI, PE-TR, APC, AF647, AF700 |
Software
Each cytometer has its own acquisition software, specifically designed to optimally run the machine and acquire data. All files can be saved as general format FCS files and further analyzed with Beckman Coulter Kaluza analysis software available for use in the CORE.
Training and Scheduling Use
Contact the Research Supervisor to arrange training. Training is for instrument use only. Please do not bring experimental samples to training without prior approval.
Use of the CORE facility is primarily for trained users although the Research Supervisor may be requested to operate the systems. Schedule planned usage on the calendars located by each piece of equipment.
Rates (Effective January 1, 2019)
Equipment | WSU | Non-WSU |
---|---|---|
Galios | $45/hr | $50/hr |
Accuri | $40/hr | $45/hr |
S3 Cell Sorter | $100/hr | $110/hr |
Computer Use Only | $5/hr | $6/hr |
Technical Support | $45/hr | $50/hr |
Training | $45/hr | $50/hr |
Misuse Fee | To Cover Cost of Cleaning/Repair | To Cover Cost of Cleaning/Repair |