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Research

Selected frames from Inscopix recorded videos of AAV transfected GCaMP6f fluorescent calcium indicator in cortical astrocytes showing the relative calcium induced fluorescence activity during different phases of the sleep cycle.

Inscopix recording of AAV transfected GCaMP6f fluorescent calcium indicator in astrocytes. Notice that calcium concentrations inside these cells spike when the sleeping mouse transitions from NREM to REM and from NREM to Wake.

Timelapse imaging of AAV transfected GCaMP6f fluorescent calcium indicator in a cortical astrocyte.

Timelapse imaging of AAV transfected CaMKII neurons with GCaMP6f fluorescent calcium indicator in mouse V1. In this experiment, different spatial gratings are presented via an LCD monitor to the awake un-anesthetized mouse during 2-photon imaging. Neurons have been pseudo-colored according to which spatial grating they respond to.