On sabbatical, and thanks to Christian Yarber’s M.S. thesis work, I was puzzling through how one could detect pathogens in the huge trade of aquatic animals. I ended up sorting out the statistical side of the question in this new manuscript:
Brunner, J. L. 2020. Pooled samples and eDNA-based detection can facilitate the “clean trade” of aquatic animals. Scientific Reports 10:10280.
I has generated a little bit of press, too, which is fun, but I am hoping that it generates interest from the pet industry, USFWS, and others interested in minimizing the risk of pathogen emergence and spillover.
This is a neat series of studies that collectively suggest that the stress-susceptibility hypothesis—chronic physiological stressors can be immunocompromising and lead to more and more deadly epidemics—works, but not exactly in the way we’d expected. It is also one of very few examples where all of the links in the logic are spelled out. Congratulations Emily! (Note: Emily is now a Postdoc with Louis Rollins-Smith at Vanderbilt.)
Emily M. Hall, Jesse L. Brunner, Brandon Hutzenbiler and Erica J. Crespi (2020) Salinity stress increases the severity of ranavirus epidemics in amphibian populations. Proc. R. Soc. B.28720200062
I was fortunate to participate in a great day-long symposium on Batrachochytrium salamandrivorans (Bsal) at The Wildlife Society / American Fisheries Society conference in Reno, Nevada last week. It was organized by Matt Gray and Jamie Voyles and featured a lot of recent results on susceptibility, microbial and immune defenses, and even mathematical models of Bsal transmission. Very cool! Thanks Matt and Jamie!
I presented on the potential for using eDNA to screen whole shipments of animals for Bsal or other pathogens. Now I just need to submit the paper!
Our research on amphibian disease (detection, stress and susceptibility and so on) as well as that of our collaborators (Erica Crespi, Caren Goldberg, Allan Pessier, Jonah Piovia-Scott) is featured in an article in Washington State Magazine. Thanks to Rebecca Phillips for both covering such an important topic and writing such a nice piece!
[Let me note that the graduate students involved in this work are not mentioned by name, so let me highlight that Christian Yarber is working on the eDNA-based detection of Bsal in trade and Erin Keller, as well as undergraduate Madelyn Kirsch, have been working very hard on the stress-susceptibility hypothesis with ranavirus and wood frogs. Thank you!!!]
It has been years in the works, but we finally published a paper describing the dynamics of ranavirus infections in bullfrogs (mostly tadpoles) and detection efficiencies with non-lethal methods. This work was supported by the Association of Zoo & Aquariums and (funds provided by the Disney Conservation Fund) and a Zoological Medicine & Wildlife Health Research Grant from the American Association of Zoo Veterinarians. Thanks to both of them! This work also involved numerous undergraduates, especially Anjulie Olson and Jeremy Rice! Thanks you two!
Brunner, J., A. Olson, J. Rice, S. Meiners, S. Le, J. Cundiff, C. Goldberg, and A. Pessier. 2019. Ranavirus infection dynamics and shedding in American bullfrogs: consequences for spread and detection in trade. Diseases of Aquatic Organisms 135:135-150.
Christina Thomas successfully defended her thesis this July after her first year of Vet School. I know it was a challenge switching gears and getting back into the tick literature after a year of crammer her brain with veterinary medicine. She did a nice job. Congratulations!
Also, her thesis work, with some additional data from Emily Burton, both funded by our DoD SERDP grant with Rick Ostfeld and colleagues, was recently accepted for publication. The citation at present is:
This was a fun project with Joe Mihaljevic (NAU) and Amy Greer (Guelph, formerly in the Collins lab with me) where we put together simple models of viral growth and an immune response and fit them to data on viral titers in bullfrog tadpoles collected over time. (The data paper is still in review.) I have been wanting to model these dynamics for years, but lacked the skill set to do it well, so it was a real pleasure to see this come together.
The paper from Mitch Le Sage’s M.S. thesis is now available online. It’s a nice set of studies, done with the help of Bailey Towey, looking at how scavenging invertebrates remove carcasses and thus can prevent pathogen transmission. Plus, pretty figures. Nice one, Mitch!
Le Sage, M. J., B. D. Towey, and J. L. Brunner. 2019. Do scavengers prevent or promote disease transmission? The effect of invertebrate scavenging on Ranavirus transmission. Functional Ecology
Johnna Eilers has been working with the Utah Division of Wildlife Resources for the last several years doing any number of cool wildlife projects in remote areas. Fortunately for us, she has decided that she’d like to be in charge of her own research project and so will be joining my and Jeb Owen’s labs this fall to start her MS research on Dermacentor andersoni ecology. We’re looking forward to her arrival!
Brendalis Camacho just graduated from the Rochester Institute of Technology and joins us for a summer REU to work on viral replication rates as a function of temperature. She is is wonderfully proficient with cell culture, so she is showing Jesse how things should be done. Welcome Brendalis!