RNA quality and quantity
Submitting high-quality RNA is the single most important factor in library success. Please read our RNA isolation guidelines [ link to pdf https://s3.wp.wsu.edu/uploads/sites/3142/2024/03/csrna_sampling.pdf ] and follow these recommendations. If you need an accessible version, or can’t access this pdf, please please contact Sascha.Duttke@wsu.edu.
- Recommended input: 1-10 µg total RNA per sample (High RNA amount allows better capture of weak enhancers)
- Minimum input: 500 ng (accepted on a case-by-case basis — contact us first)
- Maximum input: 25 µg (more RNA generally improves outcomes)
- Recommended RIN: best ≥ 7 (min 3)
RNA isolation method
Use reagent-based methods (e.g., TRIzol, TRIzol LS, QIAzol) or miRNA-compatible extraction kits. Standard spin-column kits are NOT recommended — they inefficiently retain small RNAs, which are the target of csRNA-seq. Please remember that csRNA-seq captures the regulatory state right at the moment of cell lysis/cold stop.
Supported species
csRNA-seq is applicable to any species with an annotated reference genome or transcriptome. We have experience with human, mouse, Drosophila, yeast, and plant samples. Contact us to confirm compatibility for less common organisms.
BEFORE you ship
- Complete the required sample submission form (attached to email).
- Schedule a pre-submission consultation if you have not already done so.
- Confirm biosafety classification with your institution if working with BSL-2 material.
- Ship RNA on dry ice with completed form included in the package.
We are located at Washington State University in Pullman, WA. Remote sample submission is welcome — please contact us for shipping instructions.
Contact us
All service requests begin with a free consultation. Please reach out before submitting samples — this helps us ensure your project is a good fit and that your samples are prepared optimally.
Let’s get started
Email us with a brief description of your project, including
- organism/tissue
- sample type (fresh/frozen/fixed/purified RNA)
- number of samples, and
- your research question.
We will respond within 2–3 business days.
More info about csRNA-seq on our Protocols page.