csRNA-seq
This method captures active transcription, and thus all currently made RNAs including unstable RNAs from total RNA. Please see our paper for more detail.
A csRNA-seq tutorial is available on the HOMER or check out our csRNA-seq paper
To download our latest capped small (cs)RNA-seq protocol
To download our Guidelines for collecting csRNA-seq samples
To download our the csRNA-seq reagent list
A video detailing an older version of our csRNA-seq protocol can be found on YouTube
! Note, we no longer do the bead cleanup after the frist round of CIP as quickCIP is now thermostable!
All protocols are provided as PDFs. If you need an accessible version of any document, please contact sascha.duttke@wsu.edu – we’re happy to help where we can :-).
Want more?
Here an intro video from our csRNA-seq course
And another one discussing csRNA-seq analysis