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Tools and Resources Environmental DNA

Environmental DNA References

Relevant publications

This list is updated periodically but last update was January 2018

Adamson, E. A. S., and D. A. Hurwood. 2015. Molecular ecology and stock identification. Pages 811–829Freshwater Fisheries Ecology. John Wiley & Sons, Ltd, Chichester, UK.

Adrian-Kalchhauser, I., and P. Burkhardt-Holm. 2016. An eDNA assay to monitor a globally invasive fish species from flowing freshwater. PLoS ONE 11:e0147558.

Agersnap, S., W. B. Larsen, S. W. Knudsen, D. Strand, P. F. Thomsen, M. Hesselsøe, P. B. Mortensen, T. Vrålstad, and P. R. Møller. 2017. Monitoring of noble, signal and narrow-clawed crayfish using environmental DNA from freshwater samples. PLoS ONE 12:e0179261.

Aizu, M., S. Seino, T. Sado, and M. Miya. (n.d.). Environmental DNA metabarcoding with MiFish primer reveals marine fish fauna of Tsushima Island, Nagasaki for establishing a marine protected area.

Alison A. Coulter*, D. K., J. J. Amberg‡, E. J. B. A. Reuben, and R. Goforth. 2013. Phenotypic plasticity in the spawning traits of bigheaded carp (Hypophthalmichthys spp.) in novel ecosystems. Freshwater Biology 58:1029–1037.

Amberg, J. J., S. Grace McCalla, E. Monroe, R. Lance, K. Baerwaldt, and M. P. Gaikowski. 2015. Improving efficiency and reliability of environmental DNA analysis for silver carp. Journal of Great Lakes Research 41:367–373.

Andruszkiewicz, E. A., L. M. Sassoubre, A. B. Boehm, D. Lodge, G. Lamberti, E. Willerslev, and A. Mahon. 2017. Persistence of marine fish environmental DNA and the influence of sunlight. PLoS ONE 12:e0185043.

Andruszkiewicz, E. A., H. A. Starks, F. P. Chavez, L. M. Sassoubre, B. A. Block, and A. B. Boehm. 2017. Biomonitoring of marine vertebrates in Monterey Bay using eDNA metabarcoding. PLoS ONE 12:e0176343.

Apothéloz-Perret-Gentil, L., A. Cordonier, F. Straub, J. Iseli, P. Esling, and J. Pawlowski. 2017. Taxonomy-free molecular diatom index for high-throughput eDNA biomonitoring. Molecular Ecology Resources.

Ardura, A., A. Zaiko, Y. J. Borrell, A. Samuiloviene, and E. Garcia-Vazquez. 2017. Novel tools for early detection of a global aquatic invasive, the zebra mussel Dreissena polymorpha. Aquatic Conservation: Marine and Freshwater Ecosystems 27:165–176.

Ardura, A., A. Zaiko, J. L. Martinez, A. Samuiloviene, Y. Borrell, and E. Garcia-Vazquez. 2015. Environmental DNA evidence of transfer of North Sea molluscs across tropical waters through ballast water. Journal of Molluscan Studies 81:495–501.

Ardura, A., A. Zaiko, J. L. Martinez, A. Samulioviene, A. Semenova, and E. Garcia-Vazquez. 2015. eDNA and specific primers for early detection of invasive species – A case study on the bivalve Rangia cuneata, currently spreading in Europe. Marine Environmental Research 112:48–55.

Atkinson, S., J. E. L. Carlsson, B. Ball, D. Egan, M. Kelly-Quinn, K. Whelan, and J. Carlsson. 2017. A quantitative PCR based environmental DNA assay for detecting Atlantic salmon (Salmo salar L.). bioRxiv.

Balasingham, K. 2016. Environmental dna detection as an effective tool for delimiting spatial distribution of rare and invasive species, and assessing community in flowing, freshwater ecosystems. Electronic Theses and Dissertations.

Balasingham, K. D., R. P. Walter, and D. D. Heath. 2016. Residual eDNA detection sensitivity assessed by quantitative real-time PCR in a river ecosystem. Molecular Ecology Resources.

Balasingham, K. D., R. P. Walter, N. E. Mandrak, and D. D. Heath. 2017. Environmental DNA detection of rare and invasive fish species in two Great Lakes tributaries. Molecular Ecology.

Balasingham, K. D., R. P. Walter, N. E. Mandrak, and D. D. Heath. 2017. Environmental DNA detection of rare and invasive fish species in two Great Lakes tributaries. Molecular Ecology.

Baldigo, B. P., L. A. Sporn, S. D. George, and J. A. Ball. 2017. Efficacy of Environmental DNA to Detect and Quantify Brook Trout Populations in Headwater Streams of the Adirondack Mountains, New York. Transactions of the American Fisheries Society 146:99–111.

Ballard, W. G. 2016. Fish biodiversity assessment of Abrams Creek, Tennessee. uga.

Banks, J. C., N. J. Demetras, I. D. Hogg, M. A. Knox, and D. W. West. 2016. Monitoring brown trout (Salmo trutta) eradication in a wildlife sanctuary using environmental DNA 41:1–13.

Barnes, M. A., and C. R. Turner. 2015. The ecology of environmental DNA and implications for conservation genetics. Conservation Genetics 17:1–17.

Barnes, M. A., C. R. Turner, C. L. Jerde, M. A. Renshaw, W. L. Chadderton, and D. M. Lodge. 2014. Environmental Conditions Influence eDNA Persistence in Aquatic Systems. Environmental Science & Technology 48:1819–1827.

Bastos Gomes, G., K. S. Hutson, J. A. Domingos, C. Chung, S. Hayward, T. L. Miller, and D. R. Jerry. 2017. Use of environmental DNA (eDNA) and water quality data to predict protozoan parasites outbreaks in fish farms. Aquaculture 479:467–473.

Bellemain, E., P. Harmony, G. Thomas, G. Francois, A. Valentini, M. Claude, and T. Dejean. 2016. Trails of river monsters: Detecting critically endangered Mekong giant catfish Pangasianodon gigas using environmental DNA. Global Ecology and Conservation 7:148–156.

Bergman, P. P. S., G. Schumer, S. Blankenship, and E. Campbell. 2016. Detection of Adult Green Sturgeon Using Environmental DNA Analysis. PLoS ONE 11:e0153500.

Biggs, J., N. Ewald, A. Valentini, C. Gaboriaud, T. Dejean, R. A. Griffiths, J. Foster, J. W. Wilkinson, A. Arnell, P. Brotherton, P. Williams, and F. Dunn. 2015. Using eDNA to develop a national citizen science-based monitoring programme for the great crested newt (Triturus cristatus). Biological Conservation 183:19–28.

Bista, I., G. R. Carvalho, K. Walsh, M. Seymour, M. Hajibabaei, D. Lallias, M. Christmas, S. Creer. 2017. Annual time-series analysis of aqueous eDNA reveals ecologically relevant dynamics of lake ecosystem biodiversity. Nature Communications 8:14087.

Blackman, R. C., D. Constable, C. Hahn, A. M. Sheard, J. Durkota, B. Hänfling, L. L. Handley, and M. Grabowski. 2017. Detection of a new non-native freshwater species by DNA metabarcoding of environmental samples—first record of Gammarus fossarum in the UK. Aquatic Invasions 12.

Bohmann, K., A. Evans, M. T. P. Gilbert, G. R. Carvalho, S. Creer, M. Knapp, D. W. Yu, and M. de Bruyn. 2014. Environmental DNA for wildlife biology and biodiversity monitoring. Trends in Ecology and Evolution 29:358–367.

Boothroyd, M. M., N. E. Mandrak, M. Fox, and C. C. Wilson. 2015. Environmental DNA detection and habitat occupancy of threatened spotted gar (Lepisosteus oculatus). GENOME 58:198.

Borrell, Y. J., L. Miralles, H. Do Huu, K. Mohammed-Geba, and E. Garcia-Vazquez. 2017. DNA in a bottle—Rapid metabarcoding survey for early alerts of invasive species in ports. PLoS ONE 12:e0183347.

Borrell, Y. J., L. Miralles, A. Mártinez-Marqués, A. Semeraro, A. Arias, C. E. Carleos, and E. García-Vázquez. 2017. Metabarcoding and post-sampling strategies to discover non-indigenous species: A case study in the estuaries of the central south Bay of Biscay. Journal for Nature Conservation.

Brandl, S., G. Schumer, B. M. Schreier, J. L. Conrad, B. May, and M. R. Baerwald. 2015. Ten real-time PCR assays for detection of fish predation at the community level in the San Francisco Estuary–Delta. Molecular Ecology Resources 15:278–284.

Brown, E. A., F. J. J. Chain, A. Zhan, H. J. MacIsaac, and M. E. Cristescu. 2016. Early detection of aquatic invaders using metabarcoding reveals a high number of non-indigenous species in Canadian ports. Diversity and Distributions.

Brozio, S., C. Manson, E. Gourevitch, T. J. Burns, M. S. Greener, J. R. Downie, and P. A. Hoskisson. 2017. Development and Application of an eDNA Method to Detect the Critically Endangered Trinidad Golden Tree Frog (Phytotriades auratus) in Bromeliad Phytotelmata. PLoS ONE 12:e0170619.

Buxton, A. S., J. J. Groombridge, and R. A. Griffiths. 2017. Is the detection of aquatic environmental DNA influenced by substrate type? PLoS ONE 12:e0183371.

Buxton, A. S., J. J. Groombridge, N. B. Zakaria, and R. A. Griffiths. 2017. Seasonal variation in environmental DNA in relation to population size and environmental factors. Scientific Reports 7:46294.

Buxton, A. S., J. J. Groombridge, N. B. Zakaria, R. A. Griffiths, K. C. Gough, J. Foster, J. Wilkinson, A. Arnett, P. Williams, and F. Dunn. 2017. Seasonal variation in environmental DNA in relation to population size and environmental factors. Scientific Reports 7:46294.

Bylemans, J., E. M. Furlan, C. M. Hardy, P. McGuffie, M. Lintermans, and D. M. Gleeson. 2016. An environmental DNA (eDNA) based method for monitoring spawning activity: a case study using the endangered Macquarie perch ( Macquaria australasica ). Methods in Ecology and Evolution.

Bylemans, J., E. M. Furlan, L. Pearce, T. Daly, and D. M. Gleeson. 2016. Improving the containment of a freshwater invader using environmental DNA (eDNA) based monitoring. Biological Invasions:1–9.

Cai, W., Z. Ma, C. Yang, L. Wang, W. Wang, G. Zhao, Y. Geng, and D. W. Yu. 2017. Using eDNA to detect the distribution and density of invasive crayfish in the Honghe-Hani rice terrace World Heritage site. PLoS ONE 12:e0177724.

Cannon, M. V, J. Hester, A. Shalkhauser, E. R. Chan, K. Logue, S. T. Small, and D. Serre. 2016. In silico assessment of primers for eDNA studies using PrimerTree and application to characterize the biodiversity surrounding the Cuyahoga River. Scientific reports 6:22908.

Carim, K. J., K. R. Christianson, K. M. McKelvey, W. M. Pate, D. B. Silver, B. M. Johnson, B. T. Galloway, M. K. Young, M. K. Schwartz. 2016. Environmental DNA Marker Development with Sparse Biological Information: A Case Study on Opossum Shrimp (Mysis diluviana). PLoS ONE 11:e0161664.

Carim, K. J., J. C. S. Dysthe, M. K. Young, K. S. McKelvey, and M. K. Schwartz. 2016. An environmental DNA assay for detecting Arctic grayling in the upper Missouri River basin, North America. Conservation Genetics Resources 8:197–199.

Carim, K. J., J. C. Dysthe, M. K. Young, K. S. McKelvey, and M. K. Schwartz. 2017. A Noninvasive Tool to Assess the Distribution of Pacific Lamprey (Entosphenus tridentatus) in the Columbia River Basin. PLoS ONE 12:e0169334.

Carim, K. J., T. M. Wilcox, M. Anderson, D. J. Lawrence, M. K. Young, K. S. McKelvey, and M. K. Schwartz. 2016. An environmental DNA marker for detecting nonnative brown trout (Salmo trutta). Conservation Genetics Resources 8:259–261.

Carim, K., T. Padgett-Stewart, T. Wilcox, M. Young, K. McKelvey, and M. Schwartz. 2015. Protocol for collecting eDNA samples from streams (v. 2.3). U.S.D.A. Forest Service, National Genomics Center for Wildlife and Fish Conservation V2.3:1–11.

Carraro, L., E. Bertuzzo, L. Mari, I. Es Fontes, H. Hartikainen, N. Strepparava, H. Schmidt-Posthaus, T. Wahli, J. Jokela, M. Gatto, A. Rinaldo, J. Bartholomew, and R. A. Norman. 2017. Integrated field, laboratory, and theoretical study of PKD spread in a Swiss prealpine river. PNAS 114:11992-11997.

Chain, F. J. J., E. A. Brown, H. J. MacIsaac, and M. E. Cristescu. 2016. Metabarcoding reveals strong spatial structure and temporal turnover of zooplankton communities among marine and freshwater ports. Diversity and Distributions 22:493–504.

Cho, A., T. Morris, C. Wilson, J. Freeland, and A. Naaum. 2016. Development of species-specific primers with potential for amplifying eDNA from imperilled freshwater unionid mussels. Genome 59:1141–1149.

Civade, R., T. Dejean, A. Valentini, and N. Roset. 2016. Spatial representativeness of environmental DNA metabarcoding signal for fish biodiversity assessment in a natural freshwater system. PLoS ONE 11:1–19.

Clusa, L., A. Ardura, S. Fernández, A. A. Roca, and E. García-Vázquez. 2017. An extremely sensitive nested PCR-RFLP mitochondrial marker for detection and identification of salmonids in eDNA from water samples. PeerJ 5:e3045.

Clusa, L., A. Ardura, F. Gower, L. Miralles, V. Tsartsianidou, A. Zaiko, and E. Garcia-Vazquez. 2016. An Easy Phylogenetically Informative Method to Trace the Globally Invasive Potamopyrgus Mud Snail from River’s eDNA. PLoS ONE 11:e0162899.

Clusa, L., L. Miralles, A. Basanta, C. Escot, and E. García-Vázquez. 2017. eDNA for detection of five highly invasive molluscs. A case study in urban rivers from the Iberian Peninsula. PLoS ONE 12:e0188126.

Collins, R. A., K. F. Armstrong, A. J. Holyoake, and S. Keeling. 2013. Something in the water: Biosecurity monitoring of ornamental fish imports using environmental DNA. Biological Invasions 15:1209–1215.

Comtet, T., A. Sandionigi, F. Viard, and M. Casiraghi. 2015. DNA (meta)barcoding of biological invasions: a powerful tool to elucidate invasion processes and help managing aliens. Biological Invasions 17:905–922.

Corlett, R. T. 2017. A bigger toolbox: biotechnology in biodiversity conservation. Trends in Biotechnology 35:55–65.

Cowart, D. A., K. R. Murphy, and C.-H. C. Cheng. 2017. Metagenomic sequencing of environmental DNA reveals marine faunal assemblages from the West Antarctic Peninsula. Marine Genomics.

Cowart, D. A., M. A. Renshaw, C. A. Gantz, J. Umek, S. Chandra, S. P. Egan, D. M. Lodge, and E. R. Larson. 2018. Development and field validation of an environmental DNA (eDNA) assay for invasive clams of the genus Corbicula. Management of Biological Invasions 9.

Creer, S., K. Deiner, S. Frey, D. Porazinska, P. Taberlet, W. K. Thomas, C. Potter, and H. M. Bik. 2016. The ecologist’s field guide to sequence-based identification of biodiversity. Methods in Ecology and Evolution.

Darling, J. a., and M. J. Blum. 2007. DNA-based methods for monitoring invasive species: a review and prospectus. Biological Invasions 9:751–765.

Darling, J. a., and A. R. Mahon. 2011. From molecules to management: Adopting DNA-based methods for monitoring biological invasions in aquatic environments. Environmental Research 111:978–988.

Davison, P. I., G. H. Copp, V. Créach, L. Vilizzi, and J. R. Britton. 2017. Application of environmental DNA analysis to inform invasive fish eradication operations. The Science of Nature 104:35.

Davison, P. I., V. Créach, W.-J. Liang, D. Andreou, J. R. Britton, and G. H. Copp. 2016. Laboratory and field validation of a simple method for detecting four species of non-native freshwater fish using eDNA. Journal of Fish Biology 89:1782–1793.

Davy, C. M., A. G. Kidd, and C. C. Wilson. 2015. Development and Validation of Environmental DNA (eDNA) Markers for Detection of Freshwater Turtles. PLoS ONE 10:e0130965.

Deiner, K., and F. Altermatt. 2014. Transport distance of invertebrate environmental DNA in a natural river. PLoS ONE 9:e88786.

Deiner, K., H. M. Bik, E. Mächler, M. Seymour, A. Lacoursière-Roussel, F. Altermatt, S. Creer, I. Bista, D. M. Lodge, N. de Vere, M. E. Pfrender, and L. Bernatchez. 2017. Environmental DNA metabarcoding: transforming how we survey animal and plant communities. Molecular Ecology.

Deiner, K., R. A. Knapp, D. M. Boiano, and B. May. 2013. Increased accuracy of species lists developed for alpine lakes using morphology and cytochrome oxidase I for identification of specimens. Molecular Ecology Resources 13:820–831.

Deiner, K., M. A. Renshaw, Y. Li, B. P. Olds, D. M. Lodge, and M. E. Pfrender. 2017. Long-range PCR allows sequencing of mitochondrial genomes from environmental DNA. Methods in Ecology and Evolution.

Deiner, K., J.-C. Walser, E. Maechler, F. Altermatt, E. Mächler, and F. Altermatt. 2015. Choice of capture and extraction methods affect detection of freshwater biodiversity from environmental DNA. Biological Conservation 183:53–63.

Dejean, T., A. Valentini, A. Duparc, S. Pellier-Cuit, F. Pompanon, P. Taberlet, and C. Miaud. 2011. Persistence of environmental DNA in freshwater ecosystems. PLoS ONE 6:8–11.

Dejean, T., A. Valentini, C. Miquel, P. Taberlet, E. Bellemain, and C. Miaud. 2012. Improved detection of an alien invasive species through environmental DNA barcoding: The example of the American bullfrog Lithobates catesbeianus. Journal of Applied Ecology 49:953–959.

de Souza, L. S., J. C. Godwin, M. A. Renshaw, E. Larson. 2016. Environmental DNA (eDNA) Detection Probability Is Influenced by Seasonal Activity of Organisms. PLoS ONE 11:e0165273.

De Ventura, L., K. Kopp, K. Seppälä, and J. Jokela. 2017. Tracing the quagga mussel invasion along the Rhine river system using eDNA markers: early detection and surveillance of invasive zebra and quagga mussels. Management of Biological Invasions 8:101–112.

Djurhuus, A., J. Port, C. J. Closek, K. M. Yamahara, O. Romero-Maraccini, K. R. Walz, D. B. Goldsmith, R. Michisaki, M. Breitbart, A. B. Boehm, and F. P. Chavez. 2017. Evaluation of Filtration and DNA Extraction Methods for Environmental DNA Biodiversity Assessments across Multiple Trophic Levels. Frontiers in Marine Science 4:314.

Doi, H., Y. Akamatsu, Y. Watanabe, M. Goto, R. Inui, I. Katano, M. Nagano, T. Takahara, and T. Minamoto. 2017. Water sampling for environmental DNA surveys by using an unmanned aerial vehicle. Limnology and Oceanography: Methods.

Doi, H., R. Inui, Y. Akamatsu, K. Kanno, H. Yamanaka, T. Takahara, and T. Minamoto. 2017. Environmental DNA analysis for estimating the abundance and biomass of stream fish. Freshwater Biology 62:30–39.

Doi, H., I. Katano, Y. Sakata, R. Souma, T. Kosuge, M. Nagano, K. Ikeda, K. Yano, and K. Tojo. 2017. Detection of an endangered aquatic heteropteran using environmental DNA in a wetland ecosystem. Royal Society Open Science 4.

Doi, H., T. Takahara, T. Minamoto, S. Matsuhashi, K. Uchii, and H. Yamanaka. 2015. Droplet digital polymerase chain reaction (PCR) outperforms real-time PCR in the detection of environmental DNA from an invasive fish species. Environmental science & technology 49:5601–8.

Doi, H., K. Uchii, S. Matsuhashi, T. Takahara, H. Yamanaka, and T. Minamoto. 2017. Isopropanol precipitation method for collecting fish environmental DNA. Limnology and Oceanography: Methods.

Dougherty, M. M., E. R. Larson, M. A. Renshaw, C. A. Gantz, S. P. Egan, D. M. Erickson, and D. M. Lodge. 2016. Environmental DNA (eDNA) detects the invasive rusty crayfish Orconectes rusticus at low abundances. Journal of Applied Ecology 53:722–732.

Douville, M., F. Gagné, C. Blaise, and C. André. 2007. Occurrence and persistence of Bacillus thuringiensis (Bt) and transgenic Bt corn cry1Ab gene from an aquatic environment. Ecotoxicology and Environmental Safety 66:195–203.

Dunker, K. J., A. J. Sepulveda, R. L. Massengill, J. B. Olsen, O. L. Russ, J. K. Wenburg, and A. Antonovich. 2017. Correction: Potential of Environmental DNA to Evaluate Northern Pike (Esox lucius) Eradication Efforts: An Experimental Test and Case Study. PLoS ONE 12:e0173837.

Dunker, K. J., A. J. Sepulveda, R. L. Massengill, J. B. Olsen, O. L. Russ, J. K. Wenburg, A. Antonovich. 2016. Potential of Environmental DNA to Evaluate Northern Pike (Esox lucius) Eradication Efforts: An Experimental Test and Case Study. PLoS ONE 11:e0162277.

Dunn, N., V. Priestley, A. Herraiz, R. Arnold, and V. Savolainen. 2017. Behavior and season affect crayfish detection and density inference using environmental DNA. Ecology and Evolution.

Dysthe, J. C., K. J. Carim, M. Ruggles, K. S. McKelvey, M. K. Young, and M. K. Schwartz. 2017. Environmental DNA assays for the sister taxa sauger (Sander canadensis) and walleye (Sander vitreus). PLoS ONE 12:e0176459.

Egan, S. P., E. Grey, B. Olds, J. L. Feder, S. T. Ruggiero, C. E. Tanner, and D. M. Lodge. 2015. Rapid Molecular Detection of Invasive Species in Ballast and Harbor Water by Integrating Environmental DNA and Light Transmission Spectroscopy. Environ. Sci. Technol. 49:4113–4121.

Eichmiller, J. J., P. G. Bajer, and P. W. Sorensen. 2014. The relationship between the distribution of common carp and their environmental DNA in a small lake. PLoS ONE 9:e112611.

Eichmiller, J. J., S. E. Best, and P. W. Sorensen. 2016. Effects of temperature and trophic state on degradation of environmental DNA in lake water. Environmental Science and Technology 50:1859–1867.

Eichmiller, J. J., L. M. Miller, and P. W. Sorensen. 2016. Optimizing techniques to capture and extract environmental DNA for detection and quantification of fish. Molecular Ecology Resources 16:56–68.

Erickson, R. A., C. M. Merkes, C. A. Jackson, R. R. Goforth, and J. J. Amberg. 2017. Seasonal trends in eDNA detection and occupancy of bigheaded carps. Journal of Great Lakes Research 43:762–770.

Erickson, R. A., C. B. Rees, A. A. Coulter, C. M. Merkes, S. G. McCalla, K. F. Touzinsky, L. Walleser, R. R. Goforth, and J. J. Amberg. 2016. Detecting the movement and spawning activity of bigheaded carps with environmental DNA. Molecular ecology resources 16:957–65.

Evans, N. T., and G. A. Lamberti. 2017. Freshwater fisheries assessment using environmental DNA: A primer on the method, its potential, and shortcomings as a conservation tool. Fisheries Research.

Evans, N. T., Y. Li, M. A. Renshaw, B. P. Olds, K. Deiner, C. R. Turner, C. L. Jerde, D. M. Lodge, G. A. Lamberti, and M. E. Pfrender. 2017. Fish community assessment with eDNA metabarcoding: effects of sampling design and bioinformatic filtering. Canadian Journal of Fisheries and Aquatic Sciences:cjfas-2016-0306.

Evans, N. T., B. P. Olds, M. A. Renshaw, C. R. Turner, Y. Li, C. L. Jerde, A. R. Mahon, M. E. Pfrender, G. A. Lamberti, and D. M. Lodge. 2015. Quantification of mesocosm fish and amphibian species diversity via environmental DNA metabarcoding. Molecular Ecology Resources 16:29–41.

Evans, N. T., P. D. Shirey, J. G. Wieringa, A. R. Mahon, and G. A. Lamberti. 2017. Comparative Cost and Effort of Fish Distribution Detection via Environmental DNA Analysis and Electrofishing. Fisheries 42:90–99.

Everett, M. V., and L. K. Park. 2017. Exploring deep-water coral communities using environmental DNA. Deep Sea Research Part II: Topical Studies in Oceanography.

Farrington, H. L., C. E. Edwards, X. Guan, M. R. Carr, K. Baerwaldt, and R. F. Lance. 2015. Mitochondrial Genome Sequencing and Development of Genetic Markers for the Detection of DNA of Invasive Bighead and Silver Carp (Hypophthalmichthys nobilis and H. molitrix) in Environmental Water Samples from the United States. PLoS ONE 10:e0117803.

Ficetola, G. F., C. Miaud, F. Pompanon, and P. Taberlet. 2008. Species detection using environmental DNA from water samples. Biology Letters 4:423–425.

Ficetola, G. F., J. Pansu, A. Bonin, E. Coissac, C. Giguet-Covex, M. De Barba, L. Gielly, C. M. Lopes, F. Boyer, F. Pompanon, G. Rayé, and P. Taberlet. 2014. Replication levels, false presences and the estimation of the presence/absence from eDNA metabarcoding data. Molecular Ecology Resources 15:543–556.

Ficetola, G. F., P. Taberlet, and E. Coissac. 2016. How to limit false positives in environmental DNA and metabarcoding? Molecular Ecology Resources 16:604–607.

Foote, A. D., P. F. Thomsen, S. Sveegaard, M. Wahlberg, J. Kielgast, L. a. Kyhn, A. B. Salling, A. Galatius, L. Orlando, and M. T. P. Gilbert. 2012. Investigating the Potential Use of Environmental DNA (eDNA) for Genetic Monitoring of Marine Mammals. PLoS ONE 7:2–7.

Foppen, J. W., C. Orup, R. Adell, V. Poulalion, and S. Uhlenbrook. 2011. Using multiple artificial DNA tracers in hydrology. Hydrological Processes 25:3101–3106.

Forsström, T., and A. Vasemägi. 2016. Can environmental DNA (eDNA) be used for detection and monitoring of introduced crab species in the Baltic Sea? Marine Pollution Bulletin 109:350–355.

Freeland, J. 2016. The importance of molecular markers and primer design when characterizing biodiversity from environmental DNA (eDNA). Genome.

Fujiwara, A., S. Matsuhashi, H. Doi, S. Yamamoto, and T. Minamoto. 2016. Use of environmental DNA to survey the distribution of an invasive submerged plant in ponds. Freshwater Science 35:748–754.

Fukumoto, S., A. Ushimaru, and T. Minamoto. 2015. A basin-scale application of environmental DNA assessment for rare endemic species and closely related exotic species in rivers: a case study of giant salamanders in Japan. Journal of Applied Ecology 52:358–365.

Furlan, E. M., and D. Gleeson. 2016. Improving reliability in environmental DNA detection surveys through enhanced quality control. Marine and Freshwater Research.

Furlan, E. M., and D. Gleeson. 2016. Environmental DNA detection of redfin perch, Perca fluviatilis. Conservation Genetics Resources 8:115–118.

Furlan, E. M., D. Gleeson, C. M. Hardy, and R. P. Duncan. 2016. A framework for estimating the sensitivity of eDNA surveys. Molecular Ecology Resources 16:641–654.

Gargan, L. M., T. Morato, C. K. Pham, J. A. Finarelli, J. E. L. Carlsson, and J. Carlsson. 2017. Development of a sensitive detection method to survey pelagic biodiversity using eDNA and quantitative PCR: a case study of devil ray at seamounts. Marine Biology 164:112.

Geerts, A. N., P. Boets, S. Van Den Heede, P. Goethals, and C. Van Der Heyden. 2017. A search for standardized protocols to detect alien invasive crayfish based on environmental DNA (eDNA): A lab and field evaluation. Ecological Indicators 84:564–572.

Gingera, T. D., R. Bajno, M. F. Docker, and J. D. Reist. 2017. Environmental DNA as a detection tool for zebra mussels Dreissena polymorpha (Pallas, 1771) at the forefront of an invasion event in Lake Winnipeg, Manitoba, Canada. Management of Biological Invasions 8.

Gingera, T. D., T. B. Steeves, D. A. Boguski, S. Whyard, W. Li, and M. F. Docker. 2016. Detection and identification of lampreys in Great Lakes streams using environmental DNA. Journal of Great Lakes Research 42:649–659.

Goldberg, C. S., D. S. Pilliod, R. S. Arkle, and L. P. Waits. 2011. Molecular detection of vertebrates in stream water: A demonstration using rocky mountain tailed frogs and Idaho giant salamanders. PLoS ONE 6:e22746.

Goldberg, C. S., A. Sepulveda, A. Ray, J. Baumgardt, and L. P. Waits. 2013. Environmental DNA as a new method for early detection of New Zealand mudsnails ( Potamopyrgus antipodarum ). Freshwater Science 32:792–800.

Goldberg, C. S., K. M. Strickler, and D. S. Pilliod. 2015. Moving environmental DNA methods from concept to practice for monitoring aquatic macroorganisms. Biological Conservation 183:1–3.

Goldberg, C. S., C. R. Turner, K. Deiner, K. E. Klymus, P. F. Thomsen, M. A. Murphy, S. F. Spear, A. McKee, S. J. Oyler-McCance, R. S. Cornman, M. B. Laramie, A. R. Mahon, R. F. Lance, D. S. Pilliod, K. M. Strickler, L. P. Waits, A. K. Fremier, T. Takahara, J. E. Herder, and P. Taberlet. 2016. Critical considerations for the application of environmental DNA methods to detect aquatic species. Methods in Ecology and Evolution 7:1299–1307.

Gomes, G. B., K. S. Hutson, J. A. Domingos, C. Chung, S. Hayward, T. L. Miller, and D. R. Jerry. 2017. Use of environmental DNA (eDNA) and water quality data to predict protozoan parasites outbreaks in fish farms. Aquaculture.

Gorički, Š., D. Stanković, A. Snoj, M. Kuntner, W. R. Jeffery, P. Trontelj, M. Pavićević, Z. Grizelj, M. Năpăruş-Aljančič, and G. Aljančič. 2017. Environmental DNA in subterranean biology: range extension and taxonomic implications for Proteus. Scientific Reports 7:45054.

Graham, S. 2016. Predicting Risk to Estuary Water Quality and Patterns of Benthic Environmental DNA in Queensland, Australia using Bayesian Networks. WWU Masters Thesis Collection.

Gribik, Z. 2017. Developing an eDNA System to Detect and Monitor the Spread of the Invasive Round Goby (Neogobius melanostomus) in the Waterways of Northwestern Pennsylvania. Allegheny College.

Guilfoyle, M. P., H. L. Farrington, R. F. Lance, K. C. Hanson-Dorr, B. S. Dorr, and R. A. Fischer. 2017. Movement of Hypophthalmichthys DNA in the Illinois River Watershed by the Double-Crested Cormorant ( Phalacrocorax auritus ). Waterbirds 40:63–68.

Guillera-Arroita, G., J. J. Lahoz-Monfort, A. R. van Rooyen, A. R. Weeks, and R. Tingley. 2017. Dealing with false positive and false negative errors about species occurrence at multiple levels. Methods in Ecology and Evolution.

Guillera-Arroita, G., J. J. Lahoz-Monfort, A. R. van Rooyen, A. R. Weeks, and R. Tingley. 2017. Dealing with false-positive and false-negative errors about species occurrence at multiple levels. Methods in Ecology and Evolution.

Gustavson, M. S., P. C. Collins, J. A. Finarelli, D. Egan, R. Ó. Conchúir, G. D. Wightman, J. J. King, D. T. Gauthier, K. Whelan, J. E. L. Carlsson, and J. Carlsson. 2015. An eDNA assay for Irish Petromyzon marinus and Salmo trutta and field validation in running water. Journal of Fish Biology 87:1254–1262.

Hall, E. M., E. J. Crespi, C. S. Goldberg, and J. L. Brunner. 2016. Evaluating environmental DNA-based quantification of ranavirus infection in wood frog populations. Molecular Ecology Resources 16:423–433.

Hänfling, B., L. Lawson Handley, D. S. Read, C. Hahn, J. Li, P. Nichols, R. C. Blackman, A. Oliver, and I. J. Winfield. 2016. Environmental DNA metabarcoding of lake fish communities reflects long-term data from established survey methods. Molecular Ecology 25:3101–3119.

Harper, L. R., L. L. Handley, C. Hahn, N. Boonham, H. C. Rees, K. C. Gough, E. Lewis, I. P. Adams, P. Brotherton, S. Phillips, and B. Haenfling. 2017. Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of great crested newt (Triturus cristatus). bioRxiv:215897.

Hartikainen, H., D. Bass, A. G. Briscoe, H. Knipe, A. J. Green, and B. Okamura. 2016. Assessing myxozoan presence and diversity using environmental DNA. International Journal for Parasitology 46:781–792.

Hashizume, H., M. Sato, M. O. Sato, S. Ikeda, T. Yoonuan, S. Sanguankiat, T. Pongvongsa, K. Moji, and T. Minamoto. 2017. Application of environmental DNA analysis for the detection of Opisthorchis viverrini DNA in water samples. Acta Tropica 169:1–7.

Heid, C. A., J. Stevens, K. J. Livak, and P. M. Williams. 1996. Real time quantitative PCR. Genome research 6:986–94.

Herder, J., A. Valentini, E. Bellemain, T. Dejean, J. van Delft, P. F. Thomsen, and P. Taberlet. 2014. Environmental DNA – a review of the possible applications for the detection of (invasive) species. Nijmegen, the Netherlands.

Hinlo, R., E. Furlan, L. Suitor, D. Gleeson, and M. A. Barnes. 2017. Environmental DNA monitoring and management of invasive fish: comparison of eDNA and fyke netting. Management of Biological Invasions 8:89–100.

Hinlo, R., D. Gleeson, M. Lintermans, and E. Furlan. 2017. Methods to maximise recovery of environmental DNA from water samples. PLoS ONE 12:e0179251.

Hoffmann, C., G. Schubert, and S. Calvignac-Spencer. 2016. Aquatic biodiversity assessment for the lazy. Molecular Ecology 25:846–848.

Hunter, M. E., R. M. Dorazio, J. S. S. Butterfield, G. Meigs-Friend, L. G. Nico, and J. A. Ferrante. 2016. Detection limits of quantitative and digital PCR assays and their influence in presence-absence surveys of environmental DNA. Molecular Ecology Resources.

Hunter, M. E., S. J. Oyler-McCance, R. M. Dorazio, J. A. Fike, B. J. Smith, C. T. Hunter, R. N. Reed, and K. M. Hart. 2015. Environmental DNA (eDNA) sampling improves occurrence and detection estimates of invasive Burmese pythons. PLoS ONE 10:e0121655.

Ikeda, K., H. Doi, K. Tanaka, T. Kawai, and J. N. Negishi. 2016. Using environmental DNA to detect an endangered crayfish Cambaroides japonicus in streams. Conservation Genetics Resources 8:231.

Ishige, T., M. Miya, M. Ushio, T. Sado, M. Ushioda, K. Maebashi, R. Yonechi, P. Lagan, and H. Matsubayashi. 2017. Tropical-forest mammals as detected by environmental DNA at natural saltlicks in Borneo. Page Biological Conservation.

Iversen, L. L., J. Kielgast, and K. Sand-Jensen. 2015. Monitoring of animal abundance by environmental DNA — An increasingly obscure perspective: A reply to Klymus et al., 2015. Biological Conservation 192:479–480.

Jane, S. F., T. M. Wilcox, K. S. McKelvey, M. K. Young, M. K. Schwartz, W. H. Lowe, B. H. Letcher, and A. R. Whiteley. 2015. Distance, flow and PCR inhibition: eDNA dynamics in two headwater streams. Molecular Ecology Resources 15:216–227.

Janosik, A. M., and C. E. Johnston. 2015. Environmental DNA as an effective tool for detection of imperiled fishes. Environmental Biology of Fishes 98:1889–1893.

Janzen, F. (n.d.). The detection and quantification of aquatic reptilian environmental DNA | Department of Ecology, Evolution, and Organismal Biology (EEOB).

Jeffrey B. Olsen, Cara J. Lewis, Robert L. Massengill, Kristine J. Dunker, J. K. W. 2016. Erratum to: An evaluation of target specificity and sensitivity of three qPCR assay for detecting environmental DNA from Northern Pike (Esox lucius). Conservation Genetics Resources 8:89–89.

Jerde, C. L., W. L. Chadderton, A. R. Mahon, M. a Renshaw, J. Corush, M. L. Budny, S. Mysorekar, and D. M. Lodge. 2013. Detection of Asian carp DNA as part of a Great Lakes basin-wide surveillance program. Canadian Journal of Fisheries and Aquatic Sciences 70:522–526.

Jerde, C. L., and A. R. Mahon. 2015. Improving confidence in environmental DNA species detection. Molecular Ecology Resources 15:461–463.

Jerde, C. L., A. R. Mahon, W. L. Chadderton, and D. M. Lodge. 2011. “Sight-unseen” detection of rare aquatic species using environmental DNA. Conservation Letters 4:150–157.

Jerde, C. L., B. P. Olds, A. J. Shogren, E. A. Andruszkiewicz, A. R. Mahon, D. Bolster, and J. L. Tank. 2016. Influence of Stream Bottom Substrate on Retention and Transport of Vertebrate Environmental DNA. Environmental Science & Technology 50:8770–8779.

Jiang, L., and Y. Yang. 2017. Visualization of international environmental DNA research. Current Science 112.

Jo, T., H. Murakami, R. Masuda, M. Sakata, S. Yamamoto, and T. Minamoto. 2017. Rapid degradation of longer DNA fragments enables the improved estimation of distribution and biomass using environmental DNA. Molecular Ecology Resources.

Katano, I., K. Harada, H. Doi, R. Souma, T. Minamoto, and W. Lowe. 2017. Environmental DNA method for estimating salamander distribution in headwater streams, and a comparison of water sampling methods. PLoS ONE 12:e0176541.

Keller, S. R., R. H. Hilderbrand, M. K. Shank, and M. Potapova. 2017. Environmental DNA genetic monitoring of the nuisance freshwater diatom, Didymosphenia geminata , in eastern North American streams. Diversity and Distributions.

Kelly, R., R. Gallego, and E. Jacobs-Palmer. 2017. The effect of tides on nearshore environmental DNA. PeerJ.

Kelly, R. P. 2016. Making environmental DNA count. Molecular Ecology Resources 16:10–12.

Kelly, R. P., C. J. Closek, J. L. O’Donnell, J. E. Kralj, A. O. Shelton, and J. F. Samhouri. 2017. Genetic and Manual Survey Methods Yield Different and Complementary Views of an Ecosystem. Frontiers in Marine Science 3:283.

Kelly, R. P., J. a. Port, K. M. Yamahara, and L. B. Crowder. 2014. Using environmental DNA to census marine fishes in a large mesocosm. PLoS ONE 9:e86175.

Kelly, R. P., J. a. Port, K. M. Yamahara, R. G. Martone, N. Lowell, P. F. Thomsen, M. E. Mach, M. Bennett, E. Prahler, M. R. Caldwell, and L. B. Crowder. 2014. Harnessing DNA to improve environmental management. Science 344:1455–1456.

Keskin, E. 2014. Detection of invasive freshwater fish species using environmental DNA survey. Biochemical Systematics and Ecology 56:68–74.

Keskin, E., E. M. Unal, and H. H. Atar. 2016. Detection of rare and invasive freshwater fish species using eDNA pyrosequencing: Lake Iznik ichthyofauna revised. Biochemical Systematics and Ecology 67:29–36.

Kim, E., B. Sprung, S. Duhamel, C. Filardi, and M. Kyoon Shin. 2016. Oligotrophic lagoons of the South Pacific Ocean are home to a surprising number of novel eukaryotic microorganisms. Environmental Microbiology.

Klobucar, S. L., T. W. Rodgers, and P. Budy. 2017. At the forefront: evidence of the applicability of using environmental DNA to quantify the abundance of fish populations in natural lentic waters with additional sampling considerations. Canadian Journal of Fisheries and Aquatic Sciences:1–5.

Klymus, K. E., N. T. Marshall, C. A. Stepien, A. Gooday, H. Yamamoto, P. Chevaldonné, T. Cedhagen, G. Carvalho, and S. Creer. 2017. Environmental DNA (eDNA) metabarcoding assays to detect invasive invertebrate species in the Great Lakes. PLoS ONE 12:e0177643.

Klymus, K. E., C. A. Richter, D. C. Chapman, and C. Paukert. 2015. Quantification of eDNA shedding rates from invasive bighead carp Hypophthalmichthys nobilis and silver carp Hypophthalmichthys molitrix. Biological Conservation 183:77–84.

Klymus, K. E., C. A. Richter, D. C. Chapman, and C. Paukert. 2015. A reply to Iversen et al.’s comment “Monitoring of animal abundance by environmental DNA — An increasingly obscure perspective.” Biological Conservation 192:481–482.

Klymus, K., C. Richter, N. Thompson, and J. Hinck. 2017. Metabarcoding of Environmental DNA Samples to Explore the Use of Uranium Mine Containment Ponds as a Water Source for Wildlife. Diversity 9:54.

Koizumi, N., T. Takahara, T. Minamoto, H. Doi, A. Mori, K. Watabe, and T. Takemura. 2015. Preliminary Experiment for Detection Method of Fish Inhabiting Agricultural Drainage Canals Using Environmental DNA. 農業農村工学会論文集 83:IV_7-IV_8.

Koprivnikar, J., and P. Johnson. 2015. Development and application of an eDNA method to detect and quantify a pathogenic parasite in aquatic ecosystems Development and application of an eDNA method to detect and quantify a pathogenic parasite in aquatic ecosystems. Ecological Applications 25:991–1002.

Kranzfelder, P., T. Ekrem, and E. Stur. 2016. Trace DNA from insect skins: a comparison of five extraction protocols and direct PCR on chironomid pupal exuviae. Molecular ecology resources 16:353–63.

Lacoursière-Roussel, A., G. Côté, V. Leclerc, and L. Bernatchez. 2015. Quantifying relative fish abundance with eDNA: a promising tool for fisheries management. Journal of Applied Ecology:1–10.

Lacoursière-Roussel, A., Y. Dubois, E. Normandeau, and L. Bernatchez. 2016. Improving herpetological surveys in eastern North America using the environmental DNA method. Genome 56:99–1007.

Lacoursière-Roussel, A., M. Rosabal, and L. Bernatchez. 2016. Estimating fish abundance and biomass from eDNA concentrations: variability among capture methods and environmental conditions. Molecular Ecology Resources 16:1401–1414.

LaGrange, S. 2016. Multiple stressor effects on ranavirus transmission and susceptibility. Southern Illinois University.

Lahoz-Monfort, J. J., G. Guillera-Arroita, and R. Tingley. 2016. Statistical approaches to account for false-positive errors in environmental DNA samples. Molecular Ecology Resources 16:673–685.

Laramie, M. B., D. S. Pilliod, and C. S. Goldberg. 2015. Characterizing the distribution of an endangered salmonid using environmental DNA analysis. Biological Conservation 183:29–37.

Laramie, M. B., D. S. Pilliod, C. S. Goldberg, and K. M. Strickler. 2015. Environmental DNA sampling protocol – filtering water to capture DNA from aquatic organisms. Page U.S Geological Survey Techniques and Methods.

Laroche, O., S. A. Wood, L. A. Tremblay, G. Lear, J. I. Ellis, and X. Pochon. 2017. Metabarcoding monitoring analysis: the pros and cons of using co-extracted environmental DNA and RNA data to assess offshore oil production impacts on benthic communities. PeerJ 5:e3347.

Larson, E. R., M. A. Renshaw, C. A. Gantz, J. Umek, S. Chandra, D. M. Lodge, and S. P. Egan. 2017. Environmental DNA (eDNA) detects the invasive crayfishes Orconectes rusticus and Pacifastacus leniusculus in large lakes of North America. Hydrobiologia:1–13.

Lee, P. L. M. 2017. DNA amplification in the field: move over PCR, here comes LAMP. Molecular Ecology Resources 17:138–141.

Lim, N. K. M., Y. C. Tay, A. Srivathsan, J. W. T. Tan, J. T. B. Kwik, B. Baloğlu, R. Meier, and D. C. J. Yeo. 2016. Next-generation freshwater bioassessment: eDNA metabarcoding with a conserved metazoan primer reveals species-rich and reservoir-specific communities. Royal Society Open Science 3.

Lodge, D. M., C. R. Turner, C. L. Jerde, M. a. Barnes, L. Chadderton, S. P. Egan, J. L. Feder, A. R. Mahon, and M. E. Pfrender. 2012. Conservation in a cup of water: Estimating biodiversity and population abundance from environmental DNA. Molecular Ecology 21:2555–2558.

Lopes, C. M., T. Sasso, A. Valentini, T. Dejean, M. Martins, K. R. Zamudio, and C. F. B. Haddad. 2017. eDNA metabarcoding: a promising method for anuran surveys in highly diverse tropical forests. Molecular Ecology Resources.

Lugg, W. H., J. Griffiths, A. R. van Rooyen, A. R. Weeks, and R. Tingley. 2017. Optimal survey designs for environmental DNA sampling. Methods in Ecology and Evolution.

Ma, H., K. Stewart, S. Lougheed, J. Zheng, Y. Wang, and J. Zhao. 2016. Characterization, optimization, and validation of environmental DNA (eDNA) markers to detect an endangered aquatic mammal. Conservation Genetics Resources:1–8.

MacDonald, A. J., and S. D. Sarre. 2016. A framework for developing and validating taxon-specific primers for specimen identification from environmental DNA. Molecular Ecology Resources.

Macher, J.-N., and F. Leese. 2017. Environmental DNA metabarcoding of rivers: Not all eDNA is everywhere, and not all the time. bioRxiv.

Mächler, E., K. Deiner, F. Spahn, and F. Altermatt. 2015. Fishing in the water: effect of sampled water volume on environmental DNA-based detection of macroinvertebrates. Environmental Science & Technology 50:305–312.

Mächler, E., K. Deiner, P. Steinmann, F. Altermatt, E. Maechler, K. Deiner, P. Steinmann, F. Altermatt, E. Mächler, K. Deiner, P. Steinmann, and F. Altermatt. 2014. Utility of environmental DNA for monitoring rare and indicator macroinvertebrate species. Freshwater Science 33:1174–1183.

Maggi, F. 2013. The settling velocity of mineral, biomineral, and biological particles and aggregates in water. Journal of Geophysical Research: Oceans 118:2118–2132.

Mahon, A. R., and C. L. Jerde. 2016. Using Environmental DNA for Invasive Species Surveillance and Monitoring. Pages 131–142Marine Genomics.

Mahon, A. R., C. L. Jerde, M. Galaska, J. L. Bergner, W. L. Chadderton, D. M. Lodge, M. E. Hunter, and L. G. Nico. 2013. Validation of eDNA Surveillance Sensitivity for Detection of Asian Carps in Controlled and Field Experiments. PLoS ONE 8:1–6.

Mahon, A. R., L. R. Nathan, and C. L. Jerde. 2014. Meta-genomic surveillance of invasive species in the bait trade. Conservation Genetics Resources 6:563–567.

Mahon,  a. R., M. a. Barnes, F. Li, S. P. Egan, C. E. Tanner, S. T. Ruggiero, J. L. Feder, and D. M. Lodge. 2012. DNA-based species detection capabilities using laser transmission spectroscopy. Journal of The Royal Society Interface 10:20120637–20120637.

Martellini, A., P. Payment, and R. Villemur. 2005. Use of eukaryotic mitochondrial DNA to differentiate human, bovine, porcine and ovine sources in fecally contaminated surface water. Water Research 39:541–548.

Maruyama, A., K. Nakamura, H. Yamanaka, M. Kondoh, and T. Minamoto. 2014. The Release Rate of Environmental DNA from Juvenile and Adult Fish. PLoS ONE 9:e114639.

Matsuhashi, S., H. Doi, A. Fujiwara, S. Watanabe, and T. Minamoto. 2016. Evaluation of the Environmental DNA Method for Estimating Distribution and Biomass of Submerged Aquatic Plants. PLoS ONE 11:e0156217.

Mauvisseau, Q., A. Coignet, C. Delaunay, F. Pinet, D. Bouchon, and C. Souty-Grosset. 2017. Environmental DNA as an efficient tool for detecting invasive crayfishes in freshwater ponds. Hydrobiologia:1–13.

Mawdsley, J., and A. Choudhury. 2015. Environmental DNA Clearinghouse: New Tools for Managers. February.

McKee, A. M., D. L. Calhoun, W. J. Barichivich, S. F. Spear, C. S. Goldberg, and T. C. Glenn. 2015. Assessment of environmental DNA for detecting presence of imperiled aquatic amphibian species in isolated wetlands. Journal of Fish and Wildlife Management 6:498–510.

McKee, A. M., S. F. Spear, and T. W. Pierson. 2015. The effect of dilution and the use of a post-extraction nucleic acid purification column on the accuracy, precision, and inhibition of environmental DNA samples. Biological Conservation 183:70–76.

McKelvey, K. S., M. K. Young, W. L. Knotek, K. J. Carim, T. M. Wilcox, T. M. Padgett-Stewart, M. K. Schwartz, T. M. Padgett‐Stewart, and M. K. Schwartz. 2016. Sampling large geographic areas for rare species using environmental DNA: a study of bull trout Salvelinus confluentus occupancy in western Montana. Journal of Fish Biology 88:1215–1222.

Merkes, C. M., S. G. McCalla, N. R. Jensen, M. P. Gaikowski, and J. J. Amberg. 2014. Persistence of DNA in Carcasses, Slime and Avian Feces May Affect Interpretation of Environmental DNA Data. PLoS ONE 9:e113346.

Minamoto, T., M. Fukuda, K. R. Katsuhara, A. Fujiwara, S. Hidaka, S. Yamamoto, K. Takahashi, and R. Masuda. 2017. Environmental DNA reflects spatial and temporal jellyfish distribution. PLoS ONE 12:e0173073.

Minamoto, T., T. Naka, K. Moji, and A. Maruyama. 2015. Techniques for the practical collection of environmental DNA: filter selection, preservation, and extraction. Limnology 17:23–32.

Minamoto, T., K. Uchii, T. Takahara, T. Kitayoshi, T. Satsuki, H. Yamanaka, and H. Doi. 2016. Nuclear internal transcribed spacer-1 as a sensitive genetic marker for environmental DNA studies in common carp Cyprinus carpio. Molecular Ecology Resources.

Minamoto, T., H. Yamanaka, T. Takahara, M. N. Honjo, and Z. Kawabata. 2012. Surveillance of fish species composition using environmental DNA. Limnology 13:193–197.

Miralles, L., E. Dopico, F. Devlo-Delva, and E. Garcia-Vazquez. 2016. Controlling populations of invasive pygmy mussel (Xenostrobus securis) through citizen science and environmental DNA. Marine Pollution Bulletin 110:127–132.

Miya, M., Y. Sato, T. Fukunaga, T. Sado, K. Sato, T. Minamoto, S. Yamamoto, H. Yamanaka, H. Araki, M. Kondoh, and W. Iwasaki. 2015. MiFish, a set of universal primers for metabarcoding environmental DNA from fishes: detection of > 230 species from aquarium tanks and coral reefs in the subtropical western North Pacific. GENOME 58:257.

Mizumoto, H., H. Urabe, T. Kanbe, M. Fukushima, and H. Araki. 2017. Establishing an environmental DNA method to detect and estimate the biomass of Sakhalin taimen, a critically endangered Asian salmonid. Limnology:1–9.

Mosher, B. A., K. P. Huyvaert, T. Chestnut, J. L. Kerby, J. D. Madison, and L. L. Bailey. 2017. Design- and model-based recommendations for detecting and quantifying an amphibian pathogen in environmental samples. Ecology and Evolution.

Moyer, G. R., E. Díaz-Ferguson, J. E. Hill, and C. Shea. 2014. Assessing environmental DNA detection in controlled lentic systems. PLoS ONE 9:e103767.

Muha, T. P., M. Rodríguez-Rey, M. Rolla, and E. Tricarico. 2017. Using Environmental DNA to Improve Species Distribution Models for Freshwater Invaders. Frontiers in Ecology and Evolution 5:158.

Nathan, L. M., M. Simmons, B. J. Wegleitner, C. L. Jerde, and A. R. Mahon. 2014. Quantifying Environmental DNA Signals for Aquatic Invasive Species Across Multiple Detection Platforms. Environmental Science & Technology 48:12800–12806.

Nathan, L. R., C. L. Jerde, M. L. Budny, and A. R. Mahon. 2015. The use of environmental DNA in invasive species surveillance of the Great Lakes commercial bait trade. Conservation Biology 29:430–439.

Newton, J., A. Sepulveda, K. Sylvester, and R. Thum. 2015. Potential utility of environmental DNA for early detection of Eurasian watermilfoil (Myriophyllum spicatum). Journal of Aquatic Plant Management 54:46–49.

Niemiller, M. L., M. L. Porter, J. Keany, H. Gilbert, D. W. Fong, D. C. Culver, C. S. Hobson, K. D. Kendall, M. A. Davis, and S. J. Taylor. 2017. Evaluation of eDNA for groundwater invertebrate detection and monitoring: a case study with endangered Stygobromus (Amphipoda: Crangonyctidae). Conservation Genetics Resources:1–11.

O’Donnell, J. L., R. P. Kelly, A. O. Shelton, J. F. Samhouri, N. C. Lowell, and G. D. Williams. 2016. Spatial distribution of environmental DNA in a nearshore marine habitat.

Olds, B. P., C. L. Jerde, M. A. Renshaw, Y. Li, N. T. Evans, C. R. Turner, K. Deiner, A. R. Mahon, M. A. Brueseke, P. D. Shirey, M. E. Pfrender, D. M. Lodge, and G. A. Lamberti. 2016. Estimating species richness using environmental DNA. Ecology and Evolution 6:4214–4226.

Olsen, J. B., C. J. Lewis, R. L. Massengill, K. J. Dunker, and J. K. Wenburg. 2015. An evaluation of target specificity and sensitivity of three qPCR assays for detecting environmental DNA from Northern Pike (Esox lucius). Conservation Genetics Resources 7:615–617.

Olson, Z. H., J. T. Briggler, and R. N. Williams. 2012. An eDNA approach to detect eastern hellbenders (Cryptobranchus a. alleganiensis) using samples of water. Wildlife Research 39:629–636.

Padgett-Stewart, T. M., T. M. Wilcox, K. J. Carim, K. S. McKelvey, M. K. Young, and M. K. Schwartz. 2016. An eDNA assay for river otter detection: a tool for surveying a semi-aquatic mammal. Conservation Genetics Resources 8:5–7.

Pedersen, M. W., S. Overballe-Petersen, L. Ermini, C. D. Sarkissian, J. Haile, M. Hellstrom, J. Spens, P. F. Thomsen, K. Bohmann, E. Cappellini, I. B. Schnell, N. a. Wales, C. Caroe, P. F. Campos,  a. M. Z. Schmidt, M. T. P. Gilbert,  a J. Hansen, L. Orlando, and E. Willerslev. 2014. Ancient and modern environmental DNA. Philosophical Transactions of the Royal Society B: Biological Sciences 370:20130383–20130383.

Peñarrubia, L., C. Alcaraz, A. bij de Vaate, N. Sanz, C. Pla, O. Vidal, and J. Viñas. 2016. Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples. Scientific Reports 6:39067.

Peters, L., S. Spatharis, M. A. Dario, I. J. T. Roca, A. Kintner, O. Kanstad-Hanssen, M. S. Llewellyn, and K. Praebel. 2017. Environmental DNA: a new low-cost monitoring tool for pathogens in salmonid aquaculture. bioRxiv:215483.

Piaggio, A. J., R. M. Engeman, M. W. Hopken, J. S. Humphrey, K. L. Keacher, W. E. Bruce, and M. L. Avery. 2014. Detecting an elusive invasive species: a diagnostic PCR to detect Burmese python in Florida waters and an assessment of persistence of environmental DNA. Molecular Ecology Resources 14:374–380.

Pierson, T. W., A. M. McKee, S. F. Spear, J. C. Maerz, C. D. Camp, and T. C. Glenn. 2016. Detection of an Enigmatic Plethodontid Salamander Using Environmental DNA. Copeia 104:78–82.

Piggott, M. P. 2016. Evaluating the effects of laboratory protocols on eDNA detection probability for an endangered freshwater fish. Ecology and Evolution 6:2739–2750.

Piggott, M. P. 2017. An environmental DNA assay for detecting Macquarie perch, Macquaria australasica. Conservation Genetics Resources:1–3.

Pilliod, D. S., C. S. Goldberg, R. S. Arkle, and L. P. Waits. 2013. Estimating occupancy and abundance of stream amphibians using environmental DNA from filtered water samples. Canadian Journal of Fisheries and Aquatic Sciences 70:1123–1130.

Pilliod, D. S., C. S. Goldberg, R. S. Arkle, and L. P. Waits. 2014. Factors influencing detection of eDNA from a stream-dwelling amphibian. Molecular Ecology Resources 14:109–116.

Pilliod, D. S., C. S. Goldberg, M. B. Laramie, and L. P. Waits. 2012. Application of Environmental DNA for Inventory and Monitoring of Aquatic Species. Page U.S. Geological Survey Fact Sheet 2012-3146.

Pilliod, D. S., and M. B. Laramie. 2016. Salmon redd identification using environmental DNA (eDNA). Page Open-File Report.

Pochon, X., A. Zaiko, L. M. Fletcher, O. Laroche, and S. A. Wood. 2017. Wanted dead or alive? Using metabarcoding of environmental DNA and RNA to distinguish living assemblages for biosecurity applications. PLoS ONE 12:e0187636.

Le Port, A., D. Jerry, and D. Burrows. 2017. Environmental DNA (eDNA) survey of tilapia at Meeberrie Crossing, Murchison River (Midwest Region, Western Australia).

Port, J. A., J. L. O’Donnell, O. C. Romero-Maraccini, P. R. Leary, S. Y. Litvin, K. J. Nickols, K. M. Yamahara, and R. P. Kelly. 2016. Assessing vertebrate biodiversity in a kelp forest ecosystem using environmental DNA. Molecular Ecology 25:527–541.

Qu, C., and K. A. Stewart. 2017. Comparing conservation monitoring approaches: traditional and environmental DNA tools for a critically endangered mammal. PeerJ Preprints.

Rees, H. C., K. Bishop, D. J. Middleditch, J. R. M. Patmore, B. C. Maddison, and K. C. Gough. 2014. The application of eDNA for monitoring of the Great Crested Newt in the UK. Ecology and Evolution 4:4023–4032.

Rees, H. C., K. C. Gough, D. J. Middleditch, J. R. M. Patmore, and B. C. Maddison. 2015. Applications and limitations of measuring environmental DNA as indicators of the presence of aquatic animals. Journal of Applied Ecology 52:827–831.

Rees, H. C., B. C. Maddison, D. J. Middleditch, J. R. M. Patmore, and K. C. Gough. 2014. The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology. Journal of Applied Ecology 51:1450–1459.

Renan, S., S. Gafny, R. G. B. Perl, U. Roll, Y. Malka, M. Vences, and E. Geffen. 2017. Living quarters of a living fossil – Uncovering the current distribution pattern of the rediscovered Hula painted frog ( Latonia nigriventer ) using environmental DNA. Molecular Ecology.

Renshaw, M. a., B. P. Olds, C. L. Jerde, M. M. McVeigh, and D. M. Lodge. 2014. The room temperature preservation of filtered environmental DNA samples and assimilation into a phenol-chloroform-isoamyl alcohol DNA extraction. Molecular Ecology Resources 15:168–176.

Richardson, M. F., C. D. H. Sherman, R. S. Lee, N. J. Bott, and A. J. Hirst. 2016. Multiple dispersal vectors drive range expansion in an invasive marine species. Molecular Ecology.

Robson, H. L. A., T. H. Noble, R. J. Saunders, S. K. A. Robson, D. W. Burrows, and D. R. Jerry. 2016. Fine-tuning for the tropics: application of eDNA technology for invasive fish detection in tropical freshwater ecosystems. Molecular Ecology Resources 16:922–932.

Rodgers, T. W., and K. E. Mock. 2015. Drinking water as a source of environmental DNA for the detection of terrestrial wildlife species. Conservation Genetics Resources 7:693–696.

Rodgers, T. W., J. R. Olson, S. L. Klobucar, and K. E. Mock. 2017. Quantitative PCR assays for detection of five Alaskan fish species: Lota lota, Salvelinus alpinus, Salvelinus malma, Thymallus arcticus, and Cottus cognatus from environmental DNA. bioRxiv.

Roussel, J.-M., J.-M. Paillisson, A. Tréguier, and E. Petit. 2015. The downside of eDNA as a survey tool in water bodies. Journal of Applied Ecology 52:823–826.

Sakata, M. K., N. Maki, H. Sugiyama, and T. Minamoto. 2017. Identifying a breeding habitat of a critically endangered fish, Acheilognathus typus, in a natural river in Japan. The Science of Nature 104:100.

Sansom, B. J., and L. M. Sassoubre. 2017. Environmental DNA (eDNA) shedding and decay rates to model freshwater mussel eDNA transport in a river. Environmental Science & Technology:acs.est.7b05199.

Santas, A. J., T. Persaud, B. A. Wolfe, J. M. Bauman, A. J. Santas, T. Persaud, B. A. Wolfe, and J. M. Bauman. 2013. Noninvasive Method for a Statewide Survey of Eastern Hellbenders Cryptobranchus alleganiensis Using Environmental DNA. International Journal of Zoology 2013:1–6.

Sasso, T., C. M. Lopes, A. Valentini, T. Dejean, K. R. Zamudio, C. F. B. Haddad, and M. Martins. 2017. Environmental DNA characterization of amphibian communities in the Brazilian Atlantic forest: Potential application for conservation of a rich and threatened fauna. Biological Conservation 215:225–232.

Sassoubre, L. M., K. M. Yamahara, L. D. Gardner, B. A. Block, and A. B. Boehm. 2016. Quantification of Environmental DNA (eDNA) Shedding and Decay Rates for Three Marine Fish. Environmental Science & Technology 50:10456–10464.

Sato, H., Y. Sogo, H. Doi, and H. Yamanaka. 2017. Usefulness and limitations of sample pooling for environmental DNA metabarcoding of freshwater fish communities. Scientific Reports 7:14860.

Schmelzle, M. C., and A. P. Kinziger. 2016. Using occupancy modeling to compare environmental DNA to traditional field methods for regional-scale monitoring of an endangered aquatic species. Molecular Ecology Resources 16:895–908.

Schmidt, B. R., M. Kéry, S. Ursenbacher, O. J. Hyman, and J. P. Collins. 2013. Site occupancy models in the analysis of environmental DNA presence/absence surveys: a case study of an emerging amphibian pathogen. Methods in Ecology and Evolution 4:646–653.

Schneider, J., A. Valentini, T. Dejean, F. Montarsi, P. Taberlet, O. Glaizot, and L. Fumagalli. 2016. Detection of Invasive Mosquito Vectors Using Environmental DNA (eDNA) from Water Samples. PLoS ONE 11:e0162493.

Schnell, I. B., K. Bohmann, and M. T. P. Gilbert. 2015. Tag jumps illuminated – reducing sequence-to-sample misidentifications in metabarcoding studies. Molecular Ecology Resources 15:1289–1303.

Schultz, M. T., and R. F. Lance. 2015. Modeling the Sensitivity of Field Surveys for Detection of Environmental DNA (eDNA). PLoS ONE 10:e0141503.

Scriver, M., A. Marinich, C. Wilson, and J. Freeland. 2015. Development of species-specific environmental DNA (eDNA) markers for invasive aquatic plants. Aquatic Botany 122:27–31.

Secondi, J., T. Dejean, A. Valentini, B. Audebaud, and C. Miaud. 2016. Detection of a global aquatic invasive amphibian, Xenopus laevis, using environmental DNA. Amphibia-Reptilia 37:131–136.

Serrao, N. R., S. M. Reid, and C. C. Wilson. 2017. Establishing detection thresholds for environmental DNA using receiver operator characteristic (ROC) curves. Conservation Genetics Resources:1–8.

Shaw, J. L.-A. 2015. Metagenomic amplicon sequencing as a rapid and high-throughput tool for aquatic biodiversity surveys. University of Adelaide.

Shaw, J. L. A., L. J. Clarke, S. D. Wedderburn, T. C. Barnes, L. S. Weyrich, and A. Cooper. 2016. Comparison of environmental DNA metabarcoding and conventional fish survey methods in a river system. Biological Conservation 197:131–138.

Shaw, J. L. A., L. Weyrich, and A. Cooper. 2016. Using environmental (e)DNA sequencing for aquatic biodiversity surveys: a beginner’s guide. Marine and Freshwater Research.

Shelton, A. O., J. L. O’Donnell, J. F. Samhouri, N. Lowell, G. D. Williams, and R. P. Kelly. 2016. A framework for inferring biological communities from environmental DNA. Ecological Applications.

Shogren, A. J., J. L. Tank, E. A. Andruszkiewicz, B. Olds, C. Jerde, and D. Bolster. 2016. Modelling the transport of environmental DNA through a porous substrate using continuous flow-through column experiments. Journal of the Royal Society, Interface / the Royal Society 13:423–425.

Shogren, A. J., J. L. Tank, E. Andruszkiewicz, B. Olds, A. R. Mahon, C. L. Jerde, and D. Bolster. 2017. Controls on eDNA movement in streams: Transport, Retention, and Resuspension. Scientific Reports 7:5065.

Sigsgaard, E. E., H. Carl, P. R. Møller, and P. F. Thomsen. 2015. Monitoring the near-extinct European weather loach in Denmark based on environmental DNA from water samples. Biological Conservation 183:46–52.

Sigsgaard, E. E., I. B. Nielsen, S. S. Bach, E. D. Lorenzen, D. P. Robinson, S. W. Knudsen, M. W. Pedersen, M. Al Jaidah, L. Orlando, E. Willerslev, P. R. Møller, and P. F. Thomsen. 2016. Population characteristics of a large whale shark aggregation inferred from seawater environmental DNA. Nature Ecology & Evolution.

Sigsgaard, E. E., I. B. Nielsen, H. Carl, M. A. Krag, S. W. Knudsen, Y. Xing, T. H. Holm-Hansen, P. R. Møller, and P. F. Thomsen. 2017. Seawater environmental DNA reflects seasonality of a coastal fish community. Marine Biology 164:128.

Simmons, M., A. Tucker, W. L. Chadderton, C. L. Jerde, and A. R. Mahon. 2016. Active and passive environmental DNA surveillance of aquatic invasive species. Canadian Journal of Fisheries and Aquatic Sciences 73:76–83.

Simpfendorfer, C., P. Kyne, T. Noble, J. Goldsbury, R. Basiita, R. Lindsay, A. Shields, C. Perry, and D. Jerry. 2016. Environmental DNA detects Critically Endangered largetooth sawfish in the wild. Endangered Species Research 30:109–116.

Simpson, T., P. J. Dias, M. Snow, J. Muñoz, and T. Berry. 2017. Real-time PCR detection of Didemnum perlucidum (Monniot, 1983) and Didemnum vexillum (Kott, 2002) in an applied routine marine biosecurity context. Molecular Ecology Resources 17:443–453.

Smart, A. S., R. Tingley, A. R. Weeks, A. R. van Rooyen, and M. A. McCarthy. 2015. Environmental DNA sampling is more sensitive than a traditional survey technique for detecting an aquatic invader. Ecological Applications 25:1944–1952.

Smart, A. S., A. R. Weeks, A. R. van Rooyen, A. Moore, M. A. McCarthy, and R. Tingley. 2016. Assessing the cost-efficiency of environmental DNA sampling. Methods in Ecology and Evolution.

Somervuo, P., D. W. Yu, C. C. Y. Xu, Y. Ji, J. Hultman, H. Wirta, and O. Ovaskainen. 2017. Quantifying uncertainty of taxonomic placement in DNA barcoding and metabarcoding. Methods in Ecology and Evolution 8:398–407.

Sommeria-Klein, G., L. Zinger, P. Taberlet, E. Coissac, J. Chave. 2016. Inferring neutral biodiversity parameters using environmental DNA data sets. Scientific Reports 6:35644.

Song, J. 2017. Making Sense of the Noise: Statistical Analysis of Environmental DNA Sampling for Invasive Asian Carp Monitoring Near the Great Lakes.

Song, J. W., M. J. Small, and E. A. Casman. 2017. Making sense of the noise: The effect of hydrology on silver carp eDNA detection in the Chicago area waterway system. Science of The Total Environment 605:713–720.

Spangler, M. A., F. Huettmann, I. C. Herriott, and J. A. López. 2017. Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA. Conservation Genetics Resources:1–3.

Spear, S. F., J. D. Groves, L. a. Williams, and L. P. Waits. 2015. Using environmental DNA methods to improve detectability in a hellbender (Cryptobranchus alleganiensis) monitoring program. Biological Conservation 183:38–45.

Spens, J., A. R. Evans, D. Halfmaerten, S. W. Knudsen, M. E. Sengupta, S. S. T. Mak, E. E. Sigsgaard, and M. Hellström. 2016. Comparison of capture and storage methods for aqueous macrobial eDNA using an optimized extraction protocol: advantage of enclosed filter. Methods in Ecology and Evolution.

Stat, M., M. J. Huggett, R. Bernasconi, J. D. DiBattista, T. E. Berry, S. J. Newman, E. S. Harvey, and M. Bunce. 2017. Ecosystem biomonitoring with eDNA: metabarcoding across the tree of life in a tropical marine environment. Scientific Reports 7:12240.

Stein, E. D., B. P. White, R. D. Mazor, P. E. Miller, and E. M. Pilgrim. 2013. Evaluating Ethanol-based Sample Preservation to Facilitate Use of DNA Barcoding in Routine Freshwater Biomonitoring Programs Using Benthic Macroinvertebrates. PLoS ONE 8:e51273.

Stewart, K., H. Ma, J. Zheng, and J. Zhao. 2017. Using environmental DNA to assess population-wide spatiotemporal reserve use. Conservation Biology.

Stoeckle, B. C., S. Beggel, A. F. Cerwenka, E. Motivans, R. Kuehn, and J. Geist. 2017. A systematic approach to evaluate the influence of environmental conditions on eDNA detection success in aquatic ecosystems. PLoS ONE 12:e0189119.

Stoeckle, B. c., R. Kuehn, and J. Geist. 2015. Environmental DNA as a monitoring tool for the endangered freshwater pearl mussel (Margaritifera margaritifera L.): a substitute for classical monitoring approaches? Aquatic Conservation: Marine and Freshwater Ecosystems 26:1120–1129.

Stoeckle, M. Y., L. Soboleva, Z. Charlop-Powers, A. Han, A. Johnson, and S. Holmes. 2017. Aquatic environmental DNA detects seasonal fish abundance and habitat preference in an urban estuary. PLoS ONE 12:e0175186.

Strickler, K. M., A. K. Fremier, and C. S. Goldberg. 2015. Quantifying effects of UV-B, temperature, and pH on eDNA degradation in aquatic microcosms. BIOLOGICAL CONSERVATION 183:85–92.

Strobel, B., M. B. Laramie, and D. S. Pilliod. 2017. Exploring the use of Environmental DNA to Determine the Species of Salmon redds. North American Journal of Fisheries Management:02755947.2017.1335254.

Taberlet, P., E. Coissac, M. Hajibabaei, and L. H. Rieseberg. 2012. Environmental DNA. Molecular Ecology 21:1789–1793.

Takahara, T., T. Minamoto, and H. Doi. 2013. Using Environmental DNA to Estimate the Distribution of an Invasive Fish Species in Ponds. PLoS ONE 8:e56584.

Takahara, T., T. Minamoto, and H. Doi. 2015. Effects of sample processing on the detection rate of environmental DNA from the Common Carp (Cyprinus carpio). Biological Conservation 183:64–69.

Takahara, T., T. Minamoto, H. Yamanaka, H. Doi, and Z. Kawabata. 2012. Estimation of fish biomass using environmental DNA. PLoS ONE 7:3–10.

Takahashi, M. K., M. J. Meyer, C. Mcphee, J. R. Gaston, M. D. Venesky, and B. F. Case. (n.d.). Seasonal and diel signature of eastern hellbender environmental DNA. The Journal of Wildlife Management.

Thomsen, P. F., J. Kielgast, L. L. Iversen, P. R. Møller, M. Rasmussen, and E. Willerslev. 2012. Detection of a Diverse Marine Fish Fauna Using Environmental DNA from Seawater Samples. PLoS ONE 7:1–9.

Thomsen, P. F., J. Kielgast, L. L. Iversen, C. Wiuf, M. Rasmussen, M. T. P. Gilbert, L. Orlando, and E. Willerslev. 2012. Monitoring endangered freshwater biodiversity using environmental DNA. Molecular ecology 21:2565–73.

Thomsen, P. F., P. R. Møller, E. E. Sigsgaard, S. W. Knudsen, O. A. Jørgensen, and E. Willerslev. 2016. Environmental DNA from Seawater Samples Correlate with Trawl Catches of Subarctic, Deepwater Fishes. PLoS ONE 11:e0165252.

Thomsen, P. F., and E. Willerslev. 2014. Environmental DNA – An emerging tool in conservation for monitoring past and present biodiversity. Biological Conservation 183:4–18.

Torresdal, J. D., A. D. Farrell, C. S. Goldberg, M. Acevedo, E. B. Jr, and C. Park. 2017. Environmental DNA Detection of the Golden Tree Frog (Phytotriades auratus) in Bromeliads. PLoS ONE 12:e0168787.

Tréguier, A., J.-M. Paillisson, T. Dejean, A. Valentini, M. a. Schlaepfer, and J.-M. Roussel. 2014. Environmental DNA surveillance for invertebrate species: advantages and technical limitations to detect invasive crayfish Procambarus clarkii in freshwater ponds. Journal of Applied Ecology 51:871–879.

Troth, C. 2015. Detecting the Presence of White-clawed Crayfish and Signal Crayfish using Non-invasive Environmental DNA – Christopher Troth « Wyre Forest Study Group.

Tsuji, S., M. Ushio, S. Sakurai, T. Minamoto, and H. Yamanaka. 2017. Water temperature-dependent degradation of environmental DNA and its relation to bacterial abundance. PLoS ONE 12:e0176608.

Tsuji, S., H. Yamanaka, and T. Minamoto. 2016. Effects of water pH and proteinase K treatment on the yield of environmental DNA from water samples. Limnology:1–7.

Tucker, A. J., W. L. Chadderton, C. L. Jerde, M. A. Renshaw, K. Uy, C. Gantz, A. R. Mahon, A. Bowen, T. Strakosh, J. M. Bossenbroek, J. L. Sieracki, D. Beletsky, J. Bergner, and D. M. Lodge. 2016. A sensitive environmental DNA (eDNA) assay leads to new insights on Ruffe (Gymnocephalus cernua) spread in North America. Biological Invasions:1–18.

Turner, C. R., M. A. Barnes, C. C. Y. Xu, S. E. Jones, C. L. Jerde, and D. M. Lodge. 2014. Particle size distribution and optimal capture of aqueous macrobial eDNA. Methods in Ecology and Evolution 5:676–684.

Turner, C. R., D. J. Miller, K. J. Coyne, and J. Corush. 2014. Improved methods for capture, extraction, and quantitative assay of environmental DNA from Asian bigheaded carp (hypophthalmichthys spp.). PLoS ONE 9:e114329.

Turner, C. R., K. L. Uy, and R. C. Everhart. 2014. Fish environmental DNA is more concentrated in aquatic sediments than surface water. Biological Conservation 183:93–102.

Uchii, K., H. Doi, and T. Minamoto. 2016. A novel environmental DNA approach to quantify the cryptic invasion of non-native genotypes. Molecular Ecology Resources 16:415–422.

Uchii, K., H. Doi, H. Yamanaka, and T. Minamoto. 2017. Distinct seasonal migration patterns of Japanese native and non-native genotypes of common carp estimated by environmental DNA. Ecology and Evolution.

Ulibarri, R. M., S. A. Bonar, C. Rees, J. Amberg, B. Ladell, and C. Jackson. 2017. Comparing Efficiency of American Fisheries Society Standard Snorkeling Techniques to Environmental DNA Sampling Techniques. North American Journal of Fisheries Management 37:644–651.

Ushio, M., H. Fukuda, T. Inoue, K. Makoto, O. Kishida, K. Sato, K. Murata, M. Nikaido, T. Sado, Y. Sato, M. Takeshita, W. Iwasaki, H. Yamanaka, M. Kondoh, and M. Miya. 2017. Environmental DNA enables detection of terrestrial mammals from forest pond water. Molecular Ecology Resources.

Ushio, M., H. Murakami, R. Masuda, T. Sado, M. Miya, S. Sakurai, H. Yamanaka, T. Minamoto, and M. Kondoh. 2017. Quantitative monitoring of multispecies fish environmental DNA using high-throughput sequencing. bioRxiv.

Valentini, A., P. Taberlet, C. Miaud, R. Civade, J. Herder, P. F. Thomsen, E. Bellemain, A. Besnard, E. Coissac, F. Boyer, C. Gaboriaud, P. Jean, N. Poulet, N. Roset, G. H. Copp, P. Geniez, D. Pont, C. Argillier, J.-M. Baudoin, T. Peroux, A. J. Crivelli, A. Olivier, M. Acqueberge, M. Le Brun, P. R. Møller, E. Willerslev, and T. Dejean. 2016. Next-generation monitoring of aquatic biodiversity using environmental DNA metabarcoding. Molecular Ecology 25:929–942.

Veldhoen, N., J. Hobbs, G. Ikonomou, M. Hii, M. Lesperance, and C. C. Helbing. 2016. Implementation of Novel Design Features for qPCR-Based eDNA Assessment. PLoS ONE 11:e0164907.

Vences, M., M. L. Lyra, R. G. B. Perl, M. C. Bletz, D. Stanković, C. M. Lopes, M. Jarek, S. Bhuju, R. Geffers, C. F. B. Haddad, and S. Steinfartz. 2016. Freshwater vertebrate metabarcoding on Illumina platforms using double-indexed primers of the mitochondrial 16S rRNA gene. Conservation Genetics Resources:1–5.

Venter, H. J., and C. C. Bezuidenhout. 2016. DNA-based identification of aquatic invertebrates useful in the South African context? South African Journal of Science 112:4 Pages.

Vörös, J., O. Márton, B. R. Schmidt, J. T. Gál, and D. Jelić. 2017. Surveying Europe’s Only Cave-Dwelling Chordate Species (Proteus anguinus) Using Environmental DNA. PLoS ONE 12:e0170945.

Walker, D. M., J. E. Leys, K. E. Dunham, J. C. Oliver, E. E. Schiller, K. S. Stephenson, J. T. Kimrey, J. Wooten, and M. W. Rogers. 2017. Methodological considerations for detection of terrestrial small-body salamander eDNA and implications for biodiversity conservation. Molecular Ecology Resources.

Walker, S., M. Baldi Salas, D. Jenkins, T. Garner, A. Cunningham, A. Hyatt, J. Bosch, and M. Fisher. 2007. Environmental detection of Batrachochytrium dendrobatidis in a temperate climate. Diseases of Aquatic Organisms 77:105–112.

Wegleitner, B. J., C. L. Jerde, A. Tucker, W. L. Chadderton, and A. R. Mahon. 2015. Long duration, room temperature preservation of filtered eDNA samples. Conservation Genetics Resources 7:789–791.

West Virginia Academy of Science., Y., E. Merriam, A. Welsh, J. T. Petty, and J. Anderson. 2017. Proceedings of the West Virginia Academy of Science. Page Proceedings of the West Virginia Academy of Science. West Virginia Academy of Science].

Wilcox, T. M., K. J. Carim, K. S. McKelvey, M. K. Young, and M. K. Schwartz. 2015. The Dual Challenges of Generality and Specificity When Developing Environmental DNA Markers for Species and Subspecies of Oncorhynchus. PLoS ONE 10:e0142008.

Wilcox, T. M., K. S. McKelvey, M. K. Young, S. F. Jane, W. H. Lowe, A. R. Whiteley, and M. K. Schwartz. 2013. Robust Detection of Rare Species Using Environmental DNA: The Importance of Primer Specificity. PLoS ONE 8:e59520.

Wilcox, T. M., K. S. McKelvey, M. K. Young, W. H. Lowe, and M. K. Schwartz. 2015. Environmental DNA particle size distribution from Brook Trout (Salvelinus fontinalis). Conservation Genetics Resources 7:639–641.

Wilcox, T. M., K. S. McKelvey, M. K. Young, A. J. Sepulveda, B. B. Shepard, S. F. Jane, A. R. Whiteley, W. H. Lowe, and M. K. Schwartz. 2016. Understanding environmental DNA detection probabilities: A case study using a stream-dwelling char Salvelinus fontinalis. Biological Conservation 194:209–216.

Wilcox, T. M., M. K. Schwartz, K. S. McKelvey, M. K. Young, and W. H. Lowe. 2014. A blocking primer increases specificity in environmental DNA detection of bull trout (Salvelinus confluentus). Conservation Genetics Resources 6:283–284.

Williams, K. E. 2016. Molecular detection of feral pigs using environmental DNA. Colorado State University. Libraries.

Williams, K. E., K. P. Huyvaert, and A. J. Piaggio. 2016. No filters, no fridges: a method for preservation of water samples for eDNA analysis. BMC research notes 9:298.

Williams, K. E., K. P. Huyvaert, A. J. Piaggio, R. Duncan, C. Luo, and R. Poretsky. 2017. Clearing muddied waters: Capture of environmental DNA from turbid waters. PLoS ONE 12:e0179282.

Williams, K. E., K. P. Huyvaert, K. C. Vercauteren, A. J. Davis, and A. J. Piaggio. 2017. Detection and persistence of environmental DNA from an invasive, terrestrial mammal. Ecology and Evolution 8:688–695.

Williams, M. R., R. D. Stedtfeld, C. Engle, P. Salach, U. Fakher, T. Stedtfeld, E. Dreelin, R. J. Stevenson, J. Latimore, and S. A. Hashsham. 2017. Isothermal amplification of environmental DNA (eDNA) for direct field-based monitoring and laboratory confirmation of Dreissena sp. PLoS ONE 12:e0186462.

Willoughby, J. R., B. K. Wijayawardena, M. Sundaram, R. K. Swihart, and A. J. DeWoody. 2016. The importance of including imperfect detection models in eDNA experimental design. Molecular Ecology Resources 16:837–844.

Wilson, C. C., K. M. Wozney, and C. M. Smith. 2016. Recognizing false positives: synthetic oligonucleotide controls for environmental DNA surveillance. Methods in Ecology and Evolution 7:23–29.

Wilson, C., E. Wright, J. Bronnenhuber, F. MacDonald, M. Belore, and B. Locke. 2014. Tracking ghosts : combined electrofishing and environmental DNA surveillance efforts for Asian carps in Ontario waters of Lake Erie. Management of Biological Invasions 5:225–231.

Wilson, J.-J., K.-W. Sing, P.-N. Chen, and A. Zieritz. 2017. Tracking the southern river terrapin ( Batagur affinis ) through environmental DNA: prospects and challenges. Mitochondrial DNA Part A:1–5.

Wittwer, C., S. Stoll, D. Strand, T. Vrålstad, C. Nowak, and M. Thines. 2017. eDNA-based crayfish plague monitoring is superior to conventional trap-based assessments in year-round detection probability. Hydrobiologia:1–11.

Wozney, K. M., and C. C. Wilson. 2017. Quantitative PCR multiplexes for simultaneous multispecies detection of Asian carp eDNA. Page Journal of Great Lakes Research.

Yamamoto, S., R. Masuda, Y. Sato, T. Sado, H. Araki, M. Kondoh, T. Minamoto, and M. Miya. 2017. Environmental DNA metabarcoding reveals local fish communities in a species-rich coastal sea. Scientific Reports 7:40368.

Yamamoto, S., K. Minami, K. Fukaya, K. Takahashi, H. Sawada, H. Murakami, S. Tsuji, H. Hashizume, S. Kubonaga, T. Horiuchi, M. Hongo, J. Nishida, Y. Okugawa, A. Fujiwara, M. Fukuda, S. Hidaka, K. W. Suzuki, M. Miya, H. Araki, H. Yamanaka, A. Maruyama, K. Miyashita, R. Masuda, T. Minamoto, and M. Kondoh. 2016. Environmental DNA as a “snapshot” of fish distribution: A case study of Japanese jack mackerel in Maizuru Bay, Sea of Japan. PLoS ONE 11:e0149786.

Yamamoto, S., K. Minami, K. Fukaya, K. Takahashi, H. Sawada, H. Murakami, S. Tsuji, H. Hashizume, S. Kubonaga, T. Horiuchi, M. Hongo, J. Nishida, Y. Okugawa, A. Fujiwara, M. Fukuda, S. Hidaka, K. W. Suzuki, M. Miya, H. Araki, H. Yamanaka, A. Maruyama, K. Miyashita, R. Masuda, T. Minamoto, and M. Kondoh. 2016. Correction: Environmental DNA as a “Snapshot” of Fish Distribution: A Case Study of Japanese Jack Mackerel in Maizuru Bay, Sea of Japan. PLoS ONE 11:e0153291.

Yamanaka, H., and T. Minamoto. 2016. The use of environmental DNA of fishes as an efficient method of determining habitat connectivity. Ecological Indicators 62:147–153.

Yamanaka, H., T. Minamoto, J. Matsuura, S. Sakurai, S. Tsuji, H. Motozawa, M. Hongo, Y. Sogo, N. Kakimi, I. Teramura, M. Sugita, M. Baba, and A. Kondo. 2017. A simple method for preserving environmental DNA in water samples at ambient temperature by addition of cationic surfactant. Limnology 18:233–241.

Yamanaka, H., H. Motozawa, S. Tsuji, R. C. Miyazawa, T. Takahara, and T. Minamoto. 2016. On-site filtration of water samples for environmental DNA analysis to avoid DNA degradation during transportation. Ecological Research 31:963–967.

Yoccoz, N. G. 2012. The future of environmental DNA in ecology. Molecular Ecology 21:2031–2038.

Zaiko, A., J. L. Martinez, A. Ardura, L. Clusa, Y. J. Borrell, A. Samuiloviene, A. Roca, and E. Garcia-Vazquez. 2015. Detecting nuisance species using NGST: Methodology shortcomings and possible application in ballast water monitoring. Marine Environmental Research July:1–9.

Zaiko, A., J. L. Martinez, J. Schmidt-Petersen, D. Ribicic, A. Samuiloviene, and E. Garcia-Vazquez. 2015. Metabarcoding approach for the ballast water surveillance – An advantageous solution or an awkward challenge? Marine Pollution Bulletin 92:25–34.