Introduction

This analysis provides a quality evaluation of the raw sequencing data and trims and filters low quality base pairs and reads.

Workflow

An overview of the QC process is shown below. Key steps include sequence quality evaluation using FastQC followed by read trimming and filtering using Trimmomatic.

The final results of the pipeline are outlined in blue.

1. An HTML formatted sequencing quality report produced by FastQC.
2. A set of cleaned FASTQ files produced by Trimmomatic.

Pricing

Our current price for this analysis is $10 per sample.

References

Please refer to these resources and tools that form an integral part of the pipeline process.

1. FastQC – A quality control tool for high throughput sequence data. https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
2. Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014 Aug 1;30(15):2114-20. doi: 10.1093/bioinformatics/btu170

Contact

Please contact us for a free consultation. We look forward to working with you on your analysis projects.

Phone: 509-368-6668

Email: Spok.GenomicsCore@wsu.edu

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