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Dr. Shulin Chen Bioprocessing & Bioproducts Engineering Lab

Accomplishments

BBEL’s accomplishments can be summarized in four categories: advancing science as reflected in publications; developing technologies as reflected in patent and invention disclosures; Services as reflected in the project reports, and contributions to the scientific community as reflected in the conference presentations. Only recent materials are listed.

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Publications   |   Technologies   |   Project Reports   |   Conference Presentations

Publications

  • Zhang, T., A. Jiang, J. Harrison, and S. Chen (In Press). Pigment Flocculation by Polyelectrolyte from Solution and Extraction Using Reversed Micellar System from Anaerobically Digested Effluent Journal of Chemical Technology and Biotechnology
  • Wang, J., Zhan, B., and Chen., (In Press). Mutants of the oleaginous yeast Yarrowia lipolytica with disruption of fatty acyl-CoA synthetase gene accumulate saturated fatty acid Process Biochemistry
  • Wang, G., Frear, C., S. Chen (In Press). Estimating Greenhouse Gas Emissions from Soil Following Liquid Manure Applications Using a Unit Response Curve Method Gepderma
  • Zheng, Y., Z. Chi., and S. Chen (In Press). Oleaginous Yeast Cryptococcus curvatus for Biofuel Production: Ammonia’s Effect Biomass and Bioenergy
  • Li, D. L. Chen; D. Xu; X. Zhang; N. Ye; F. Chen; S. Chen (In Press). Preparation and characteristics of bio-oil from Sargassum patens C. Agardh, collected from the Yellow Sea, China. Bioresource Technology
  • Yu, L., P. Wensel, S., Chen (In Press). Hydrodynamic and Kinetic study of Cellulase Production by Trichoderma reesei with Pellet MorphologyBiotechnology and Bioengineering
  • Zhao, Q., P. Wensel, S., Chen. (In Press). Alkalitalea saponilacus gen. nov., sp. nov., an obligately anaerobic, alkaliphilic, xylanolytic bacterium from Soap Lake, Washington State, USA. International Journal of Systematic and Evolutionary Microbiology
  • Ke, J., D. D. Laskar, D. Gao and S. Chen (In Press). Advanced biorefinery in lower termite – effect of combined pretreatment during the chewing process Biotechnology for Biofuels
  • Miao, C., M. Chakraborty, S. Chen (In Press). Impact of reaction conditions on the simultaneous production of polysaccharides and bio-oil from heterotrophically grown Chlorella sorokiniana by a unique sequential hydrothermal liquefaction process Bioresource Technology
  • Ke, Jing; Singh, Deepak; Chen, Shulin (In Press). Metabolism of polycyclic aromatic hydrocarbons by the wood-feeding termite Coptotermes formosanus (Shiraki) Journal of Agricultural and Food Chemistry
  • Ke, J., and S. Chen (In Press). Modulation of lignin deposition/composition via phytic acid reduction in seed improves the quality of barley straw for sugar release and ethanol production Biomass and Bioenergy
  • Yu. J., T. Tan, and S. Chen (In Press). Regulation of Central Carbon Metabolism in Recombinant Saccharomyces cerevisiae: A Whole-Cell Strategy for Efficient Xylose Fermentation Biomass and Bioenergy
  • Xiong, X. Wang, X., and S. Chen (In Press). Engineering of a Xylose Metabolic Pathway in Rhodococcus Strains for Biofuel Production Applied and Environmental Microbiology
  • Chakraborty, ,Moumita, Chao Miao, Armando McDonald,, Shulin Chen (In Press). Concomitant extraction of bio-oil and value added polysaccharides from Chlorella sorokiniana using a unique sequential hydrothermal extraction technology Fuels
  • Wang., G., and S. Chen (In Press). A Review on Parameterization and Uncertainty in Modeling Greenhouse Gas Emissions from Soil Gepderma
  • Wensel, P., L. Yu., and S. Chen (In Press). Simulation with Computational Fluid Dynamics of Succinic Acid and Co-Product Biorefinery Process Journal of Bioprocessing and Biotechniques
  • Li, Demao, Limei Chen, Shulin Chen, Xiaowen Zhang, Fangjian Chen, and Naihao Ye (2012). Comparative evaluation of the pyrolytic and kinetic characteristics of a macroalga (SARGASSUM THUNBERGII) and a freshwater plant (POTAMOGETON CRISPUS) Fuel
  • Ke, J., Singh, D., Chen, S., 2011 (2011). Aromatic compound degradation by the wood-feeding termite Coptotermes formosanus (Shiraki) International Biodeterioration and Biodegradation
  • Zeng, Jijiao; Deepak Singh; Shulin Chen (2011). Thermal decomposition kinetics of wheat straw treated by Phanerochaete chrysosporiumInternational Biodeterioration and Biodegradation
  • Ke, J., Laskar, D., Chen, S., 2011 (2011). Biodegradation of hardwood lignocellulosics by the western poplar clearwing borer, Paranthrene robiniae (Hy. Edwards) Biomacromolecules
  • Zeng, Jijiao, Deepak Singh, Shulin Chen (2011). Thermal decomposition kinetics of wheat straw treated by Phanerochaete chrysosporium.International Biodeterioration and Biodegradation
  • Yu, X. Y. Zheng, S. Chen (2011). Oil production by oleaginous yeasts using hydrolysate from pretreatment of wheat straw with dilute sulfuric acidBioresource Technology
  • Ke, J., D. Laskar, S. Chen (2011). In-situ lignocellulosic unlocking mechanism in termite for carbohydrate hydrolysis: critical lignin modificationBiotechnology for Biofuel
  • Singh, Deepak, Jijiao Zeng, Shulin Chen (2011). Increasing manganese peroxidase productivity of Phanerochaete chrysosporium by optimizing carbon sources and supplementing small molecules Letters of Applied Microbiology
  • Chi, Z., Y. Zheng, A. Jiang, and S. Chen (2011). Lipid production by culturing oleaginous yeast and algae with food waste and municipal wastewater in an Integrated Process Applied Biochemistry and Biotechnology
  • Yu, X. Y. Zheng, S. Chen (2011). Oil production by oleaginous yeasts using hydrolysate from pretreatment of wheat straw with dilute sulfuric acidBioresource Technology
  • Ke, J. S. Chen (2011). Thermal characterization of softwood lignin modification by termite Coptotermes formosanus Biomass and Bioenergy
  • Collins, H.P., J.D. Streubel, A.K. Alva, C. Frear, S. Chen, S.F. Fransen, C. Kruger and D. Granatstein (2011). Greenhouse Gas Emissions from an Irrigated Silt Loam Soil Amended with Anaerobic Digested Dairy Manure Soil Science Society of America Journal
  • Chi, Zhanyou, James V O’Fallon, Shulin Chen (2011). Bicarbonate Produced from Carbon Capture for Algae Culture Trends in Biotechnology
  • Ke, J., D. Laskar, S. Chen., (2011). In-situ lignocellulosic unlocking mechanism in termite for carbohydrate hydrolysis: critical lignin modification Biotechnology for Biofuel
  • Chi, Z., Zheng, Y., Jiang, A., Chen, S., 2011 (2011). Lipid production by culturing oleaginous yeast and algae with food waste and municipal wastewater in an integrated process. Applied Biochemistry and Biotechnology
  • Singh, D., Zeng, J., Chen, S. (2011). Increasing manganese peroxidase productivity of Phanerochaete chrysosporium by optimizing carbon sources and supplementing small molecules Letters of Applied Microbiology
  • Collins, H.P., J.D. Streubel, A.K. Alva, C. Frear, S. Chen, S.F. Fransen, C. Kruger and D. Granatstein (2011). Greenhouse Gas Emissions from an Irrigated Silt Loam Soil Amended with Anaerobic Digested Dairy Manure Soil Science Society of America Journal
  • Chi, Z., Zheng, Y., Chen, S., 2011 (2011). Oleaginous yeast Cryptococcus curvatus culture with dark fermentation hydrogen production effluent as feedstock for microbial lipid production International Journal of Hydrogen Energy
  • Chi, Zhanyou, James V O’Fallon, Shulin Chen (2011). Bicarbonate Produced from Carbon Capture for Algae Culture Trends in Biotechnology
  • Wu, Gaihong, Ronglin He, Wendi Jia, Yapeng Chao, Shulin Chen (2011). Strain improvement and process optimization of Trichderma reesei Rut C30 for enhanced cellulase production Biofuels
  • Yu, J. Singh, D. Liu, N. Lian, J., Tan, T., and Chen, S (2011). Construction of a Glucose and Xylose Co-Fermenting Industrial Saccharomyces cerevisiae by Expression of Codon-Optimized Fungal Xylose Isomerase Journal of Biobased Materials and Bioenergy
  • Zheng, Y., Zhanyou Chi, Ben Lucker, Shulin Chen (2011). Two-stage heterotrophic and phototrophic culture strategy for algal biomass and lipid production Bioresource Technologies
  • Laskar, D. D., Jing Ke, Jijiao Zeng, Xin Gao and Shulin Chen (2011). Py-GC/MS as a powerful and rapid tool for determining lignin compositional and structural changes in biological processes Current Analytical Chemistry
  • Zhang, T., Chao, y., Liu, N., Thompson, J., Garcia, M., He, B.B., Van Gerpen, J., Chen, S. (2011). Case study of biodiesel-diesel blends as a fuel in marine environment Advances in Chemical Engineering and Science
  • Wang, C., Wu, G., Chen, C., Chen, S., 2011 (2011). High Production of β-glucosidase by Aspergillus niger on Corncob Applied Biochemistry and Biotechnology
  • Guo W., Jia W., Li Y., and Chen S. (2010). Performances of Lactobacillus brevis for producing lactic acid from hydrolysate of lignocellulosics. Applied Biochemistry and Biotechnology, 161:124-136.
  • Li, Z., R. Zemetra, and S. Chen (2010). Hydrolysis and fermentation of wheat straw with lignin modification Biomass and Bioenergy
  • Ma, J. and Chen S (2010). ASBR with retained biomass on influent solids treating dairy manure: Effect of settling time Water Science and Technology
  • Li, Z., Y. Liu, Z. Chi, and S. Chen (2010). Effects of Pluronic F68 on Manganese Peroxidase Production by Pelletized Phanerochaete chrysosporium Applied Biochemistry and Biotechnology
  • Ke, J and Chen, S (2010). Aromatic compound degradation by the wood-feeding termite Coptotermes formosanus International Biodeterioration and Biodegradation
  • Ke, J., D. Laskar, S. Chen (2010). Biodegradation of hardwood lignocellulosics by the western poplar clearwing borer, Paranthrene robiniaeBiomacromolecules
  • Wang Z.W. and Chen S. (2009). Potential of biofilm-based biofuel production. Applied Microbiology and Biotechnology, 83(1):1-18.
  • Zaher U., Buffiere P., Steyer J.P., and Chen S. (2009). A procedure to estimate proximate analysis of mixed organic wastes. Water Environment Research 81(4):407-415.
  • Hu B., Zhou X., Forney L., and Chen S. (2009). Changes in microbial community composition following treatment of methanogenic granules with chloroform. Environmental Progress and Sustainable Energy, 28(1):60-71.
  • Zaher U., Li R., Jeppsson U., Steyer J.P., and Chen S. (2009). GISCOD: General Integrated Solid Waste Co-Digestion Model. Water Research, 43(10):2717-2727.
  • Zaher U., Pandy P., and Chen S. (2009). A simple elemental continuity based model to study the anaerobic microbial activity for the treatment of dairy manure. Applied Mathematical Modelling, 33(9):3553-3564.
  • Li R., Chen S., Li X., Lar J.S., He Y., and Zhu B. (2009). Anaerobic codigestion of kitchen waste with cattle manure for biogas production. Energy and Fuels, 23(4):2225-2228.
  • Gaboutloeloe G.K., Chen S., Barber M.E., and Stockle C. (2009). Combinations of horizontal and vertical flow constructed wetlands to improve nitrogen removal. Water, Air, Soil Pollution, 9:279-286.
  • Li R., Chen S., and Li X. (2009). Biogas production from anaerobic co-digestion of food waste with dairy manure in a two-phase digestion system.Applied Biochemistry and Biotechnology, 160:643-654.
  • Li R., Chen S., and Li X. (2009). Anaerobic co-digestion of kitchen waste and cattle manure for methane production. Energy Sources Part A, 31(20):1848-1856.
  • Chi Z., Liu Y., Frear C., and Chen S., (2009). Study of a two-stage growth of DHA-producing marine algae Schizochytrium limacinum SR21 with shifting dissolved oxygen level. Applied Microbiology and Biotechnology, 81(6):1141-1148.
  • Johnson R., Garcia-Perez M., Liaw S., Ha S., Lin S., and Chen S. (2009). Py-GC/MS Studies to evaluate hydro-thermal pretreatment as a way to enhance the production of sugars from the fast pyrolysis of wheat straw. Energy and Fuels, 23(12):6242-6252.
  • Yao C., Lei T., Elliot W.J., McCool D.K., Zhao J., and Chen S. (2008). Critical conditions for rill initiation. Transactions of the ASABE, 51(1):107-114.
  • Agarwal S., Powers J.R., Swanson B.G., Chen S., and Clark S. (2008). Influence of salt-to-moisture ratio on starter culture and calcium lactate crystal formation. Journal of Dairy Science, 91(8):2967-2980.
  • Liu Y., Liao W., and Chen S. (2008). Study of pellet formation of filamentous fungi Rhizopus oryzae using a multiple logistic regression model.Biotechnology and Bioengineering, 99(1):117-128.
  • Liao W., Liu Y., Frear C., Chen S. (2008). Co-production of fumaric acid and chitin from a nitrogen-rich lignocellulosic material — dairy manure – using a pelletized filamentous fungus Rhizopus oryzae ATCC 20344. Bioresource Technology, 99(13):5859-5866.
  • Wu B., Liu Y., Liao W., Chen S. (2008). A numerical model for predicting diffusivity in a biocatalyst particle with heterogeneous reactions. AIChE Journal, 54(8):2182-2189.
  • Kruger C., Chen S., MacConnell C., Harrison J., Shumway R., Zhang T., Oakley K., Bishop C., Frear C., Davidson D., Bowers K. (2008). High-quality fiber and fertilizer as co-products from anaerobic digestion. Journal of Soil and Water Conservation, 63(1):12A-13A.
  • Wu B. and Chen S. (2008). CFD simulation of non-Newtonian fluid flow in anaerobic digester. Biotechnology and Bioengineering. 99(3):700-711. Uludag-Demirer S., Demirer G.N., Frear C., and Chen S. (2008).Anaerobic digestion of diary manure with enhanced ammonia removal.Environmental Management, 86(1):193-200.
  • Demirer G.N. and Chen S. (2008). Anaerobic biogasification of undiluted dairy manure in leaching bed reactors. Waste Management, 28(1):112-119.
  • Hu B., Liu Y., Chi Z., and Chen S. (2008). Biological hydrogen production via bacteria immobilized in calcium alginate gel beads. Biological Engineering, 1(1):25-37.
  • Hu B. and Chen S. (2008). Biological hydrogen production using chloroform-treated methanogenic granules. Applied Biochemistry and Biotechnology, 148:83-95.
  • Liu Y., Liao W., and Chen S. (2008). Co-production of lactic acid and chitin using a pelletized filamentous fungus Rhizopus oryzae cultured on cull potatoes and glucose. Journal of Applied Microbiology, 105(5):1521-1528.
  • Chen S. (2008). Industrial biosystems engineering and biorefinery systems.Chinese Journal of Biotechnology, 24(6):940-945.
  • Zaher U., Frear C., Pandey P., and Chen S. (2008). Evaluation of a new fixed-bed digester design utilizing large media for flush dairy manure treatment.Bioresource Technology, 99(18):8619-8625
  • Liao W., Liu Y., Wen Z., Frear C., Chen S. (2008). Kinetic modeling of enzymatic hydrolysis of cellulose in differently pretreated fibers from dairy manure. Biotechnology and Bioengineering, 101(3):441-451.
  • Singh D., and Chen S., (2008). The white-rot fungus Phanerochaete chrysosporium: conditions for the production of lignin degrading enzymes.Applied Microbiology and Biotechnology, 81(3):399-417.
  • Liao, W., Liu, Y., Wen, Z., Frear, C., Chen, S. (2007). Studying the effects of reaction conditions on components of dairy manure and cellulose accumulation using dilute acid treatment. Bioresource Technology, 98:1992-1999.
  • Liao, W., Liu, Y., Liu., C., and S. Chen. (2006). Acid Hydrolysis of Fiber from a Dairy Manure. Bioresources Technology, 97:1687-1695.
  • Frear, C., Zhao, B., Fu, G. Richardson, M., and S., Chen. (2005). Biomass Inventory and Bioenergy Assessment – An evaluation of organic material resources for bioenergy production in Washington State. Publication N. 05-07-047, Washington Department of Ecology.
  • Wen, Z., W. Liao, and S. Chen. (2005). Production of cellulase by Trichoderma reesi from dairy manure. Bioresource Technology, 96:491-499.
  • Liao, W., Z. Wen, S. Hurley, Y. Liu, C. Liu, and S. Chen (2005). Effects of Hemicellulose, Lignin on Enzymatic Hydrolysis of Cellulose from Dairy Manure. Applied biochemistry and biotechnology, 121-124:1017-1030.
  • Chen, S., W. Liao, C. Liu, Z. Wen, R. L. Kincaid, J. H. Harrison, D. C. Elliott, and D. J. Stevens. (2005). Studies into using manure in a biorefinery concept.Applied biochemistry and biotechnology, 121-124:999-1015.
  • Wen, Z., W. Liao, and S. Chen. (2005). Production of cellulase/β-Glucosidase by the mixed fungi culture of Trichderma reesei and aspergillus phoenicis on dairy manure. Applied biochemistry and biotechnology, 121-124:93-104.
  • Wen, Z., and S. Chen. (2005). Mixed Fungal Culture for cellulase production.Process Biochemistry, 40(9), 3087-3094.
  • Wen, Z., W., Liao and S. Chen (2004). Hydrolysis of animal manure lingocellulosics for reducing sugar production. Bioresource Technology, 91:31-39.
  • Liao, W., Z. Wen, C. Liu, Y. Liu., S. Chen. (2004). Optimizing dilute acid hydrolysis of hemicellulose in a nitrogen-rich cellulosic material — dairy manure. Bioresource Technology, 94:33-41.
  • Wang, Chunli, Gaihong Wu, Chang Chen, and Shulin Chen (). High Production of β-glucosidase by Aspergillus niger on Corncob Applied Biochemistry and Biotechnology

Technologies

Chi, Z., S. Chen et al. (2008). Integrated algal nutraceutical and biodiesel process and system. 61/029,974 Filed, February, 2008.

A Two-Stage, High-Cell Density Process for Heterotrophic Omega-3-Enriched Algal Production Using Crude Glycerol and Integrated within the Biodiesel Refinery Disclosed is a two-stage, integrated, high-cell density fermentation process for production of omega-3-rich micro-algae which could then be used directly as a food and/or animal feed or further processed for isolation of the DHA or omega-3 fatty acids for nutraceutical and fuel use. The micro-algae used in the fermentation process are Schizochytrium limacinum SR 21, a known high-cell density species with unique abilities for efficient metabolism of glycerol carbon sources. A key to the process is the developed two-stage process using two separate reactors that takes advantage of the algae’s unique growth processes by controlling operating conditions in a first stage focused on cell number growth and then shifting operating conditions to induce a second stage focused on cell mass and fatty acid accumulation. The second stage occurring in the second reactor operates at two different operating conditions during its fed batch cycle so as to induce, at the end of the cyle, a higher percentage of DHA and/or omega-3 fatty acids in the fatty acid profile. Operating conditions that are controlled and shifted to induce maximum production in the two stages are carbon and nutrient feed types and concentrations, dissolved oxygen content, and temperature. The result of the combined two-stage process is a five-day fermentation regime that allows for extremely high cell densities (~150 g/L) and high productivity (~1.2 g/L hr) of algae with total, PUFA and DHA fat percentages of ~50, 25 and 20%, respectively. The entire process is efficiently integrated within the biodiesel refinery process. The main feedstock utilized by the micro-algae and for which the whole process and choice of particular organism is designed is crude glycerol produced as a by-product of the biodiesel refining process. After some pre-treatment which can be performed on-site, the crude glycerol is used as a carbon source to ferment the algae. The produced algae can then be put through a downstream separation process whereby the algae biomass is directly treated to produce: (1) omega-3 fatty acids for nutraceutical sale; (2) biodiesel from the non-omega-3 fatty acids; (3) crude glycerol that is reclaimed as carbon feed for future fermentation and algae production; and (4) algal biomass waste which can be used as an animal feed both internally in the fermentation process or externally.

Chi, Z., S. Chen et al. (2008). Heterotrophic algal high cell density production method and system 61/029,969 Filed, February, 2008.

The invention comprises a multi-phase method for high cell density heterotrophic algal growth, wherein waste products are utilized as primary carbon sources to yield omega-3-rich micro-algae for use as feeds and/or supplements for feeds.

Wang, Z.W., Frear, C., and Chen, S. (2008). Selection pressure driven biomass immobilization on influent solids for high-rate anaerobic digestion of diluted solids wastes at psychrophilic temperature. Invention disclosure filed with WSU Research Foundation, March, 2008.

Jiang, A., and S. Chen. (2008). Anaerobic digestion integrated nutrient recovery and biogas scrubbing system. 61/029,961 Filed, February, 2008.

Disclosed is a novel integrated nutrient recovery system that works in series with an on-farm anaerobic digester for removal and recovery of nitrogen (N) and phosphorous (P) in the form of ammonia sulfate solution and phosphorous-rich solids, respectively. The phosphorous-rich solids are obtained through dosing of specialized flocculants and subsequent settling and dewatering. Ammonia air stripping using a closed-loop recycle with novel tower and packing design with subsequent acid treatment for ammonia salt recovery is used for the ammonia recovery. Control of the effluent pH after ammonia treatment is accomplished by absorption of acid components in biogas that is bubbled through the effluent. The result is a pH controlled effluent and a scrubbed biogas with complete removal of hydrogen sulfide, partial removal of carbon dioxide and an associated increase in methane concentration. Placement of the unit operations is flexible within the integrated system allowing for added flexibility in operation for optimization with various types of anaerobic digested (AD) manure effluent.

Chi, Z., S. Chen et al. (2008). Integrated algal nutraceutical and biodiesel process and system. 61/029,974 Filed

Chi, Z., S. Chen et al. (2008). Heterotrophic algal high cell density production method and system. 61/029,969 Filed

Zhang, T., K. Bower, Harrison, J., and Chen, S. (2007). Compositions and methods for wastewater treatment. 60/949,479 Filed, July, 2007.

Zhang, T., K. Bower, Harrison, J., and Chen, S. (2007). Compositions and methods of wastewater treatment. 60/949,479 Filed

Liu, Y., S. Chen, and W. Liao. (2004). Co-production of Chitin/Chitosan and Lactic Acid using Rhizopus oryzae. Disclosure filed, December, 2004.

Liu, Y., S. Chen, and W. Liao. (2004). Co-production of Chitin/Chitosan and Lactic Acid using Rhizopus oryzae Disclosure filed

Project Reports

  • Zaher, U., Cheong, D.Y., Wu, B., and S. Cheh. (2007). Producing Energy and Fertilizer from Organic Mnuicipal Solids Waste. Porject report to Washsington State Depatment of Ecology.
  • Frear, C., Zhao, B., Fu, G. Richardson, M., and S., Chen. (2005). Biomass Inventory and Bioenergy Assessment – An evaluation of organic material resources for bioenergy production in Washington State. Publication N. 05-07-047, Washington Department of Ecology.
  • Chen, S., W. Liao, C. Liu, Z. Wen, R.L. Kincaid, J.H. Harrison, D.C. Elliot, A.E. Solana, M.D. Brown, and D.J., Stevens. (2003). Value-Added Chemicals form Animal Manure. Northwest Bioproduct Research Institute-Report No.1.Prepared for the U.S. Department of Energy under Contract DE-AC06-76RL01830.
  • Chen, S., C. Frear, B. Zhao, and G. Fu. (2003). Bioenergy inventory and assessment for Eastern Washington. Project report to Washington Department of Ecology. http://www.ecy.wa.gov/biblio/0307021.html

Conference Presentations

  • Zaher U., Buffiere P., Steyer J.-P., Rosen C., Jeppsson U. and Chen S. (2008).Integrated modeling tool to optimize co-digestion of solid wastes. The 23rd International Conference on Solid Waste Technology and Management. Philadelphia, PA, U.S.A, March 30 – April 2, 2008.
  • Zaher U. and Chen S. (2008). Biochemical and spatial based selection of anaerobic digestion feedstock: California and Washington case study. The 23rd International Conference on Solid Waste Technology and Management. Philadelphia, PA, U.S.A., March 30 – April 2, 2008.
  • Zaher U., Pandey P. and Chen S. (2007). A simple model to simulate the anaerobic digestion process and estimate the potential nutrient recovery from biosolids. ASABE Annual International Meeting. Minneapolis, Minnesota, USA, June 17-20, 2007.
  • Zaher U. and Chen S. (2007). Integrated modeling of wastewater treatment systems – perspectives and good modeling practice, Middle East Waste & Water Congress. Hyatt Regency Dubai, UAE, May 28-29th 2007.
  • Zaher U. and Chen S. (2007). Integrated modeling of wastewater treatment systems – perspectives and good modeling practice. Middle East Waste & Water Congress. Hyatt Regency Dubai, UAE. May 28-29th 2007.
  • Zaher U. and Chen S. (2007). Identifying state of the art in biological treatment of municipal solids waste. Middle East Waste & Water Congress. Hyatt Regency Dubai, UAE. May 28-29th 2007.
  • Chi z., Chen s. (2007). DHA production from high biomass concentration culture of schizochytrium sp. with a shift strategy. The world congress on industrial biotechnology & bioprocessing. Orlando, FL. 2007.
  • Chen, Liu, Li, Z., Wen. (2007). Fungal based on-site enzyme production for Biorefinery. International Biorefinery Conference. Beijing, China. Oct. 20-23, 2007.
  • Zaher U. and Chen S. (2007). Identifying state of the art in biological treatment of municipal solids wast, Middle East Waste & Water Congress. Hyatt Regency Dubai, UAE, May 28-29th 2007.
  • Chi Z., Chen S., (2007). DHA production from high biomass concentration culture of schizochytrium sp. with a shift strategy, Poster presentation at the world congress on industrial biotechnology and bioprocessing. Orlando, FL
  • Chen, Liu, Li, Z., Wen, (2007). Fungal based on-site enzyme production for Biorefinery, International Biorefinery Conference. Beijing, China, Oct. 20-23
  • Tianxi Zhang, Keith E. Bowers, Joseph H. Harrison and Shulin Chen (2006).Phosphorus Removal from Dairy Wastewater, Pacific Northwest Clean Water Associate Annual Conference. Coeur d’Alene, Idaho, USA, October 1-4.
  • Tianxi Zhang, Keith E. Bowers, Joseph H. Harrison and Shulin Chen (2006).Phosphorus Reduction in Dairy Effluent through Flocculation, ASABE Annual International Meeting Portland, Oregon, USA, 9-12 July 2006.
  • Bo Hu, Yan Liu, Zhanyou Chi, Shulin Chen (2006). Biological Hydrogen Production via Bacteria Immobilized in Calcium Alginate Gel Beads, 28th Symposium on Biotechnology for Fuel and Chemicals Nashville, TN.
  • Bo Hu, Yan Liu, Zhanyou Chi, Shulin Chen. (2006). Biological Hydrogen Production via Bacteria Immobilized in Calcium Alginate Gel Beads. 28th Symposium on Biotechnology for Fuel and Chemicals. Nashville, TN. 2006.
  • Liao, W., Liu, Y., Chen, S., (2006). A new approach of pellet formation of a filamentous fungus – Rhizopus oryzae, Poster presentation at the 28th symposium on biotechnology for fuels and chemicals. Nashville, TN, April 30 – May 3.
  • Liu, Y., Liao, W., Chen, S., (2006). Production of L-(+)-lactic acid from a pelletized filamentous Rhizopus oryzae culture using cull potatoes and glucose as nutrients, Poster presentation at the 28th symposium on biotechnology for fuels and chemicals. Nashville, TN, April 30 – May 3.
  • Chi, Z., Hu B., Liu Y., Chen, S., (2006). Production of Omega-3 Polyunsaturated Fatty Acids from Cull Potato Using an Algae Culture Process, Poster presentation at 28th Symposium on Biotechnology for Fuels and Chemicals. Nashville, TN
  • Zaher U. and Chen S. (2006). Interfacing the IWA anaerobic digestion model no1 (ADM1) with manure and solid waste characteristics. Water Environment Federation Technical Exhibition and Conference (WEFTEC 79th). Dallas, Texas, USA, October 21-25, 2006.
  • Zaher U., Pandey P., Rongping L., Frear C. and Chen S. (2006). An innovative model based approach to plan anaerobic digester start up and operation.Pacific Northwest Clean Water Association. Coeur d’Alene, Idaho, USA, October 1-4, 2006.
  • Liao, W., Liu, Y., Chen, S. (2006). A new approach of pellet formation of a filamentous fungus – Rhizopus oryzae. Poster presentation at the 28th symposium on biotechnology for fuels and chemicals. Nashville, TN, April 30 – May 3.
  • Liu, Y., Liao, W., Chen, S., (2005). Co-production of lactic acid from a pelletized filamentous Rhizopus oryzae culture using cull potatoes as raw material, Oral presentation at the world congress on industrial biotechnology and bioprocessing. Orlando, FL, April 19-22.
  • Liu, Y., Liao, W., Chen, S., (2005). Optimization of L-(+)-lactic acid production using pellet-form Rhizopus oryzae NRRL 395, Poster presentation at the 27th symposium on biotechnology for fuels and chemicals. Denver, CO, May 1-4.
  • Liao, W., Liu, Y., Wen, Z., Liu, C., Chen, S. (2005). Enzymatic hydrolysis of hydrogen peroxide pretreated fiber from an agricultural residue—dairy manure. Poster presentation at the 27th symposium on biotechnology for fuels and chemicals. Denver, CO, May 1-4.
  • Chunmei Yao, Tingwu, Lei., S. Chen. (1996). Rill incision on hill slopes in the Loess Plateau. ASABE International Conference Meeting. Oregon, USA, July.
  • Liao, W., Liu, Y., Chen, S., (). Fumaric acid production from a nitrogen-rich agricultural residue — dairy manure using fungal fermentation, Oral presentation at the world congress on industrial biotechnology and bioprocessing. Orlando, FL, April 19-22.