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John Peters' lab Personnel

Krista Shisler

Research Associate


Washington State University
Institute of Biological Chemistry
425 Clark Hall
Pullman, WA 99164


Research Interests


After earning my bachelor’s degree in Chemistry at the University of Wyoming, I attended graduate school at Montana State University where I received my Ph.D. in Biochemistry. My graduate work focused on bioinorganic chemistry of anaerobic metalloenzymes containing [4Fe-4S] clusters. I characterized the active site structure of radical SAM enzymes throughout various stages of catalysis. Following my Ph.D. work, I continued with bioinorganic chemistry and held a post doc position at Montana State University studying heme proteins. Keeping with the theme of iron, I am working with [FeFe] hydrogenase in the Peters lab in addition to the carboxylases 2-KPCC and acetone carboxylase. I enjoy observing metal centers with a variety of spectroscopic techniques and discovering intermediate states. When I’m not busy in the lab, I take care of my two young children and husband.



  • 2016       Ph.D. in Biochemistry, Dr. Joan Broderick, Montana State University, Bozeman, MT
  • 2009       Bachelor’s of Science, University of Wyoming, Laramie, WY

Research Experience

  • 2019-Present     Postdoctoral Research, Dr. John Peters, Washington State University, Pullman, WA
  • 2016-2017            Postdoctoral Research, Dr. Jennifer DuBois, Montana State University, Bozeman, MT



  1. The unique Phe–His dyad of 2-ketopropyl coenzyme M oxidoreductase/carboxylase selectively promotes carboxylation and S–C bond cleavage. Prussia, G.; Shisler, K.; Zadvornyy, O.; Streit, B.; DuBois, J.; Peters, J. Journal of Biological Chemistry (2021), 297:100961.
  2. Insights into the unique carboxylation reactions in the metabolism of propylene and acetone. Mus, F.; Wu, H.; Alleman, A.; Shisler, K.; Zadvornyy, O.; Bothner, B.; DuBois, J.; Peters, J. Biochemical Journal (2020), 477: 2027-2038.
  3. Monovalent Cation Activation of the Radical SAM Enzyme Pyruvate Formate-Lyase Activating Enzyme. Shisler, K.; Hutcheson, R.; Horitani, M.; Duschene, K.; Crain, A.; Byer, A.; Shepard, E.; Rasmussen, A.; Yang, J.; Broderick, W.; Vey, J.; Drennan, C.; Hoffman, B.; Broderick, J. Journal of the American Chemical Society (2017), 139: 11803-11813.
  4. Electron Spin Relaxation and Biochemical Characterization of the Hydrogenase Maturase HydF: Insights into [2Fe-2S] and [4Fe-4S] Cluster Communication and Hydrogenase Activation. Shepard, E. M.; Byer, A. S.; Aggarwal, P.; Betz, J. N.; Scott, A. G.; Shisler, K. A.; Usselman, R. J.; Eaton, G. R.; Eaton, S. S.; Broderick, J.B. Biochemistry, (2017), 56, 3234-3247.
  5. Reactions of ferrous coproheme decarboxylase (HemQ) with O2 and H2O2 yield ferric heme b. Streit, B.; Celis, A.; Shisler, K.; Rodgers, K.; Lukat-Rodgers, G.; DuBois, J. Biochemistry (2017), 56: 189-201.
  6. A Structure-Based Mechanism for Oxidative Decarboxylation Reactions Mediated by Amino Acids and Heme Propionates. Celis, A.; Gauss, G.; Streit, B.; Shisler,K.; Moraski, G.; Rodgers, K.; Lukat-Rodgers, G.; Peters, J.; DuBois, J. Journal of the American Chemical Society (2017), 139: 1900-1911.
  7. Radical SAM catalysis via an organometallic intermediate with an Fe–[5′-C]-deoxyadenosyl bond. Horitani, M.; Shisler, K.; Broderick, W.; Hutcheson, R.; Duschene, K.; Marts, A.; Hoffman, B.; Broderick, J. Science (2016), 352: 822-825.
  8. Why Nature Uses Radical S‑Adenosyl-L-methionine Enzymes so Widely: Electron Nuclear Double Resonance Studies of Lysine 2,3-Aminomutase Show the 5′-dAdo• “Free Radical” Is Never Free. Horitani, M.; Byer, A.; Shisler, K.; Chandra, T.; Broderick, J.; Hoffman, B. Journal of the American Chemical Society (2015), 137: 7111-7121.
  9. Glycyl Radical Activating Enzymes: Structure, Mechanism, and Substrate Interactions. Shisler, K.; Broderick, B. Archives of Biochemistry and Biophysics (2014), 546: 64-71.
  10. Emerging Themes in Radical SAM Chemistry. Shisler, K.; Broderick, B. Current Opinion in Structural Biology: Catalysis and Regulation Issue (2012), 22: 701-710.