July 19, 2023 – Our studied about the intrinsically disordered C-terminus of plant purinoceptor was published in FEBS Letters. Congrats to Joel on his 2nd first-authored paper! 

How to cite:
Sowders JM, Jewell JB, Tripathi D, Tanaka K (2023) The intrinsically disordered C-terminus of purinoceptor P2K1 fine-tunes plant responses to extracellular ATP. FEBS Letters

P2K1 is a plant-specific purinoceptor that perceives extracellular ATP (eATP), a signaling molecular implicated in various physiological processes. Interestingly, P2K1 harbors a C-terminal intrinsically disordered region (IDR). When we overexpressed a truncated P2K1 (P2K1t) lacking the IDR, primary root growth completely ceased in response to eATP. We investigated the functional roles of the IDR in P2K1 using a combination of molecular genetics, calcium imaging, gene expression analysis, and histochemical approaches. We found that the P2K1t variant gave rise to an amplified response to eATP, through accumulation of superoxide, altered cell wall integrity, and ultimate cell death in the primary root tip. Together, these observations underscore the significant involvement of the C-terminal tail of P2K1 in root growth regulation.