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Tissue Imaging, Metabolomics and Proteomics Laboratory TIMPL

Cutting gels

If you are going to cut out the gel bands yourself from a gel, please follow carefully the following instructions:

  • Clean the gel box or surface you are cutting the gel on/in with ethanol, isopropanol or methanol before the gel comes in contact with it.
  • Use a clean razor/scalpel blade, and clean it further with ethanol, isopropanol or methanol before cutting.

  • Handle and cut the gel in a laminar flow clean hood if one is available in your lab.
  • Cut out a maximum of 10mm of a single gel lane for each sample you will be submitting -but-
  • Don’t cut out unstained gel surrounding a band – cut tightly to the stained area.
  • Slice the cut gel band into 1mm cubes, but no smaller.
  • Place the sliced band into a high-quality Eppendorf microcentrifuge tube, which has been rinsed with 50% organic solvent and millipure water.
  • Close the tube as soon as your bands are inside!