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  • Research Associate
  • Associate Director (Proteomics/Metabolomics Service Center, Prasad Lab)


  • Ph.D. in Pharmaceutical Analysis – National Institute of Pharmaceutical Education and Research (NIPER), SAS Nagar, India
  • Master of Science in Pharmaceutical Analysis – National Institute of Pharmaceutical Education and Research (NIPER), SAS Nagar, India
  • Bachelor in Pharmaceutical Sciences – Maharshi Dayanand University, Rohtak, India

Research Interests:

  • Quantitative mass spectrometry (targeted and untargeted metabolomics)


Dr. Singh is a research associate in the Department of Pharmaceutical Sciences at the College of Pharmacy and Pharmaceutical Sciences, Washington State University, Spokane. His major focus is on employing proteomics and metabolomics-informed approaches to predict age-related variability in drug transport, metabolism, and pharmacokinetics. His professional journey includes significant stints with leading pharmaceutical companies such as Biocon Bristol Myers Squibb Research & Development Center (BBRC), and Sandoz, India, where he supported small molecule drug discovery and generic pharmaceuticals development. He specializes in mass spectrometry-based structural characterization of small and large molecules. Dr. Singh is a reviewing editor for Pharmacology Research and Perspectives (an official journal of the ASPET and the British Pharmacological Society) and an executive committee member of the Division for Drug Metabolism and Disposition under ASPET. He obtained his MS in 2012 and Ph.D. in 2020 in Pharmaceutical Sciences from NIPER, Mohali, India.


  1. Singh, D. K., Ahire, D., Davydov, D. R., & Prasad, B. (2024). Differential tissue abundance of membrane-bound drug metabolizing enzymes and transporter proteins by global proteomics. Drug Metabolism and Disposition (PMID: 38641346).
  2. Singh, D. K., Basit, A., Rettie, A. E., Alade, N., Thummel, K., & Prasad, B. (2023). Characterization of Gla proteoforms and non-Gla peptides of gamma carboxylated proteins: Application to quantification of prothrombin proteoforms in human plasma. Analytica Chimica Acta (PMID: 37996163).
  3. Subash S., Singh D.K., Ahire D.S., Khojasteh S.C., Murray B.P., Zientek M, Jones R.S., Kulkarni P., Smith B.J., Heyward S., Cronin C.N., Prasad B., 2023. Dissecting parameters contributing to the underprediction of aldehyde oxidase-mediated metabolic clearance of drugs. Drug Metabolism and Disposition. DOI:
  4. Kruger L., Lapehn S., Paquette A., Singh D.K., MacDonald J., Bammler T.K., Enquobahrie D.A., Zhao Q., Mozhui K., Sathyanarayana S., and Prasad B., 2023. Characterization of xenobiotic and steroid disposition potential of human placental tissue and cell lines (BeWo, JEG-3, JAR, and HTR-8/SVneo) by quantitative proteomics. Drug Metabolism and Disposition, 51 (6), pp.1493-1500.
  5. Sharma S., Singh D.K., Mettu V.S., Yue G., Ahire D., Basit A., Heyward S., and Prasad B., 2023 Quantitative characterization of clinically relevant drug-metabolizing enzymes and transporters in rat liver and intestinal segments for applications in PBPK modeling. Molecular Pharmaceutics, 20(3), pp. 1737–1749
  6. Sharma, S., Ahire, D., Basit, A., Lajoie, M., Wang, C., Lee, M.S., Blithe, D.L., Amory, J.K., Singh, D.K., Heyward, S. and Prasad, B., 2022. Dimethandrolone, a potential male contraceptive pill, is primarily metabolized by the highly polymorphic UDP-glucuronosyltransferase 2B17 enzyme in human intestine and liver. Drug Metabolism and Disposition, 50(12), pp.1493-1500.


  1. Singh, D. K., Ailabouni, A., Thakur, A., Paine, M. F., and Prasad, B., deconvoluting the global metabolomic changes caused by a complex clinical pharmacokinetic cimetidine-metformin interaction involving organic cation transporters. ASPET 2024 Annual Meeting, Arlington, VA, USA (May 16-19, 2024).
  2. Singh D.K., Ahire D., and Prasad B., A PheWAS and genotype-informed metabolomics approach revealed potentially novel endogenous substrates of UGT2B17. 2023 Drug Metabolism GRC Meeting, Holderness, NH, United States (July 9 – 14, 2023).
  3. Singh D.K., Mettu V.S., Thakur A., Ailabouni A.S., Naji-Talakar S., and Prasad B., High-resolution mass spectrometry coupled with XCMS online for high-throughput detection and identification of drug metabolites. ASPET 2023 Annual Meeting, St Louis, MO, USA (May 18 – 21, 2023).
  4. Singh, D.K., Ahire, D., Yue, G., and Prasad, B., Global versus targeted proteomics for quantification of DMET proteins in human liver, kidney, and intestine. ISSX/MDO 2022 Meeting, Seattle, WA (September 9 – 14, 2022).

Contact Details:

Telephone: (509) 368-6535



Office Location: Pharmaceutical & Biomedical Sciences Building, Room # PBS-322, Spokane, WA